1.
Afr. j. lab. med. (Online)
; 7(1): 1-4, 2018. ilus
Artigo
em Inglês
| AIM
| ID: biblio-1257318
RESUMO
The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories.Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation