Oxidative stress and lipid profile among hypertensive patients at a tertiary centre in Kano, Northwest, Nigeria

Background: Hypertension as a global public health challenge is a major risk factor for cardiovascular (CVD) and coronary heart diseases (CHD) because of its chronic sequelae. It is accompanied by dyslipidemia and oxidative stress leading to increase in lipid peroxidation. This study aimed to measure the fasting serum lipid profile and malondialdehyde (MDA) and determine the atherogenic index as well as the cardiovascular risk ratio among hypertensive patients in Kano, Nigeria. Patients and Methods: Two hundred subjects (100 hypertensive patients vs. 100 normotensive controls) were recruited for the study. The fasting serum lipid profile and MDA were assayed using routine laboratory methods. Lipid ratios that predict and identify an individual's increased risk for cardiovascular diseases were then determined from the results of the profile. Results: The serum total cholesterol (7.0±0.5 vs 4.1±0.4 mmol/L), triglycerides (2.9±0.2 vs 2.0±0.3 mmol/Lg/dl), LDL cholesterol (3.8±0.4 vs 2.6±0.4 mmol/L), VLDL cholesterol (3.0±0.2 vs 2.1±0.2 mmol/L) and MDA (TBARS) (9×10-5±1.4×10-5 vs 3×106±0.9×10-6 mol/l) were significantly (p<0.05) increase in hypertensive patients compared to normotensive controls. HDL cholesterol was significantly higher (p<0.05) in normotensive controls compared to hypertensive patients (31.4±8 vs 23.9±6 mg/dl). A statistically significant (p<0.05) positive correlation was observed between LDL cholesterol and MDA only. Both the atherogenic index (AI) ratio and the CardioRisk ratio were significantly higher in Hypertensives than Normal controls (10.4 vs 4.1; 11.7 vs 5.1 respectively). Conclusion: This study demonstrated an increased occurrence of atherogenic lipid profile and oxidative stress among hypertensive patients. It further showed a strong correlation between dyslipidaemia and oxidative stress. Therapeutic lifestyle changes and use of statins should be considered an integral part of the treatment for hypertensive patients in Nigeria

Oxidant status of children infected with plasmodium falciparum malaria in Katsina metropolis, Northwestern Nigeria

Background: Malaria is a global menace caused by the transfer of a plasmodium parasite to a host by an infected anopheles mosquito. Upon infection; the overwhelmed host releases free radicals which have the capacity to induce oxidative damage by lipid peroxidation. This study was undertaken to assess the effect of malaria caused by Plasmodium falciparum on some antioxidant markers and lipid peroxidation levels in children attending hospitals in Katsina State; Nigeria. Materials and Methods: Blood samples were collected from untreated subjects upon confirmation of Plasmodium falciparum parasitaemia using the Giemsa stain technique. One hundred and sixty (160) consenting individuals (80 infected patients and 80 uninfected subjects) comprising of both sexes were randomly selected. The levels of antioxidant markers and malondialdehyde (MDA) - a lipid peroxidation marker were determined. Descriptive analysis was employed using SPSS version 16.0 and significance between groups was ascertained using students' T-test. Results: P. falciparum malarial infection significantly (p 0.05) reduced the antioxidant markers [vitamins A; C; et E; and reduced glutathione (GSH)] by 65.4%; 29.7%; 48.1%; 40.4% respectively in males and by 54.2%; 36.6%; 55.7% ; 36.6% in females when compared with values obtained from uninfected; healthy children. Conversely; lipid peroxidation levels were significantly (p 0.05) higher in children with parasitaemia than in nonparasitaemic controls. Males showed greater than 200% increase; while it increased by 138% in females. Conclusion: Our findings indicate a reciprocal relationship; where high levels of lipid peroxidation correspond to low levels of antioxidants; which may be due to over utilization of the antioxidants in order to counteract the effect of free radicals. This may be responsible for oxidative stress and consequently; tissue damage associated with pathology of malaria in Nigerian children

Chlamydia Infection; Plasma Peroxidation and Obesity in Tubal Infertility

Background: Genital tract infections and obesity are both sources of oxidative stress. Alterations in immune and antioxidant parameters may arise from this or from an indeterminate autoimmune mechanism. Objective: This study aimed to investigate the association of Chlamydial infection; obesity and oxidative response with tubal infertility in Nigerian women. Methods: It was a case-control study of 40 women with tubal infertility and 32 fertile women; respectively; recruited from the Infertility and Family Planning Clinics respectively; of the University College Hospital; Ibadan; Nigeria. Anthropometric indices were measured in each subject and endocervical swabs were taken to screen for current genital tract infection. Antioxidant; hormonal and immunologic analysis were performed on serum. Results: None of the subjects had current genital tract infections. Chlamydia trachomatis IgG positivity was significantly higher in infertile than in fertile subjects [OR 4.33; 95CI (0.078-0.681)]. No significant variations were observed in the anthropometric indices; antioxidant parameters and hormones between infertile and the fertile women. Body mass index correlated positively with oxidative stress in infertile subjects. Waist and hip circumferences correlated negatively with oestradiol in women with tubal infertility. Conclusion: Chlamydial infection is associated with tubal factor infertility; however; obesity seems to increase oxidative stress and reduce fertility potential in women with tubal factor infertility

Prevention of Radiation Induced Hematological Alterations by Medicinal Plant Rosmarinus Officinalis; in Mice

The modulatory influence of Rosmarinus officinalis (rosemary) leaves extract was investigated in Swiss albino mice at a dose of 3 Gy gamma radiation. For this purpose; adult Swiss albino mice were irradiated with 3 Gy gamma rays in the presence (experimental) or absence (control) of rosemary (1000 mg/kg body wt.). These animals were necropsied and their blood was collected at days 1; 3; 5; 10; 20 and 30 post-irradiation. A decrease in the number of erythrocyte and leucocyte counts; hemoglobin content and hematocrit percentage was scored in the control group; whereas a recovery pattern was recorded in experimental animals and a normal value of hematological parameters were regained by day 30 post-treatment. In irradiated group; glutathione level was registered low in the blood; whereas a significant elevation was estimated in rosemary pre-treated animals. An increase in lipid peroxidation level above normal was evident in serum of irradiated mice; while a significant decrease in such values was noted in rosemary pretreated group. The present study suggests the possible radioprotective ability of rosemary extract

Antigenotoxic Effect of Ferulic Acid in 7;12-Dimethyl Beza (A) - Anthracene (DMBA) Induced Genotoxicity

The antigenotoxic effect of ferulic acid was carried out by evaluating the cytogenetic markers; the micronuclei frequency and chromosomal aberrations; in the bone marrow of hamsters in 7;12dimethylbenz(a)anthracene (DMBA) induced genotoxicity. Genotoxicity was induced in experimental hamsters by single intraperitoneal injection of DMBA (30mg kg-1 b.w). Pretreatment of ferulic acid orally at a dose of 40mg kg-1 b.w for five days significantly reduced the frequency of micronucleated polychromatic erythrocytes (MnPCEs) and the percentage of chromosomal aberrations in hamster's bone marrow. Our results thus suggest that ferulic acid has potent antigenotoxic effect in DMBA induced genotoxicity in golden Syrian hamsters

Evaluation of Moringa oleifera Leaf Extract on Alcohol-induced Hepatotoxicity

A study on the protective effect of Moringa oleifera leaf extract in acute alcohol-induced hepatotoxicity in rats was evaluated. Rats fed alcohol only produced significant increase in the levels of enzyme markers of tissues damage (ALT; AST and ALP); lipid peroxidation (TBARS) and decreased serum vitamin C levels compared to normal control rats. Pretreatment with 100 and 200mg/kg body weight of extract significantly decreased the levels of enzyme markers; lipid peroxidation and markedly increased serum vitamin C level in a dose-dependent manner. Post-treatment with 200mg/kg body weight of extract significantly enhanced the recovery of animals from hepatic damage compared to untreated control. Lipid peroxidation and depletion of vitamin C due to oxidative stress could be the possible mechanisms of alcohol induced toxicity and the protective effect of the extract could be as a result of its ability to inhibit lipid peroxidation and prevent the depletion of vitamins C

Determination des marqueurs de la lipoperoxydation par fluorimetrie et des vitamines antioxydantes par HPLC chez des africains residant a Bordeaux (France)

Le stress oxydant est le desequilibre entre le systeme antioxydant et le systeme radicalaire lie soit a une production excessive de radicaux libres soit a une diminution des moyens de leur degradation; entrainant ainsi des lesions biochimiques. Dans cette etude; nous nous sommes interesses a: - l'evaluation des taux plasmatiques moyens des marqueurs de la Lipoperoxydation (MDAL; MDAT et TBARS) et des vitamines Antioxydantes (vitamines A; E et B carotenes). - l'evaluation des taux moyens des vitamines antioxydantes dans les LDL.- la comparaison de tous les taux moyens a leurs valeurs usuelles d'une part; et la comparaison des taux moyens des vitamines antioxydantes dans le plasma ainsi que dans les LDL d'autre part. - une etude comparee entre les taux de TBARS des africains vivant en France et ceux vivant en cote d'Ivoire. - l'etude des correlations entre les marqueurs de la Lipoperoxydation et le sexe; l'age ainsi que les vitamines antioxydantes. Il ressort de celle etude les constats suivants: - le profil des marqueurs de la lipoperoxydation est plus eleve chez les africains vivant en France (2.08 nmol/ml) que celui de leurs compatriotes vivant en cote d'ivoire (1;12nmol/ml); d'ou l'apparition d'un stress oxydant. - l'apparition du stress oxydant est due soit a une augmentation des marqueurs de la lipoperoxydation (MDAL;MDAT et TBARS) soit a une diminution des piegeurs de radicaux libres (vitamines A; E et Beta carotenes).- le profil de la lipoperoxydation d'un individu depend de son age mais pas de son sexe