Phenotypic identification of soil bacterial and fungal communities inhabiting an archaeological monument at Augustine University, Ilara Epe, southwest Nigeria

Background: The Sungbo Eredo Monument is an ancient public work with a system of defensive walls and ditches located in Eredo Local Council Development Area of Epe, Lagos State, southwest Nigeria. A huge section of the monument cuts through the Augustine University campus, forming two-sided vertical walls with a deep ridge in-between. The objective of this investigative study is to determine the microbial profile of soil samples from the monument in the University campus. Methodology: Soil samples were collected from the topsoil at a depth of 7.5cm from four randomly selected points along the edge of the monument. The samples were transported to the microbiology laboratory of the Department of Biological Sciences of Augustine University for analysis. Samples were cultured on Nutrient agar (NA) and incubated aerobically for 24-48 hours for bacteria isolation and on Sabouraud's Dextrose agar (SDA) for 72 hours for fungi isolation. Bacterial colonies on NA were preliminarily identified to genus level by Gram reaction and conventional biochemical test scheme for Gram-positive (catalase, coagulase, starch hydrolysis) and Gram-negative isolates (oxidase, urease test, indole, methyl red, Voges Proskauer and sugar fermentation tests). Fungi colonies on SDA were identified using conventional macroscopic and microscopic characteristics. Antibiotic susceptibility test of the bacterial isolates to selected antibiotics was done using the Kirby Bauer disc diffusion method. Results: A total of twenty-three bacterial isolates in four genera; Bacillus, Staphylococcus, Cellobiococcus and Micrococcus and nine fungal isolates in three genera; Saccharomyces, Aspergillus and Botrytis were identified from the cultures. The bacterial isolates were sensitive (>50% sensitivity) to only gentamicin and ofloxacin, with 65.2% and 78.3% sensitivity rates respectively, while they were largely resistant to all other antibiotics such as ceftriaxone, erythromycin, cefuroxime, cloxacillin, ceftazidime and augmentin, with resistance rates of 65.2%, 65.2%, 73.9%, 82.6%, 86.9%, 91.3% respectively. Conclusion: The results of this investigative study revealed the presence of antibiotic-resistant bacteria (mainly Gram-positive) and fungi on the archaeological monument of Augustine University, adding to the existing data on microbial spectrum of archaeological monuments that could be useful for unraveling human cultural habits and microbe-related human diseases. However, further studies on molecular identification of these microbial spectrum will be required to ascertain their genetic relatedness and ancestral phylogeny, which will be useful for archaeologists in their study of the Sungbo-Eredo ancestral monument.

Geophysical characterization of abandoned dumpsite soil properties for pre- foundation delineation at Bowen University Campus, Iwo, Nigeria

ABSTRACT: Geophysical investigation and microstudy of soil properties were carried out within Bowen University, for the characterization of the subsurface pattern in the area using vertical electrical sounding (VES) survey and evaluation of soil properties with a view to mapping subsurface geological features, such as weak and competent zones and to determine the overburden thickness of the area for pre-foundation studies for a proposed high-rise building. Four(4) VES was carried out in the study area employing schlumberger electrode configuration. The data collected were used to curve match and the results used for subsequent interpretation to reveal the depth, thickness and resistivity of the study area. In addition, soil samples were collected from three (3) selected points located at the entrance, centre and the end of the study area at about a depth 0-60 cm. Consequently, the VES results were presented as resist graph, which revealed the range of values for the depth (1.3 ­ 24.5), thickness (1.3 ­ 15.4 m) and resistivity (357.4 - 6311.6 ohms), which was later used to generated the geoelectric maps of the study area. It was also observed that the result of the soil properties revealed that all the samples taken from the study area have low bulk density (1.41 g/cm3, 1.26 g/cm3, 1.36 g/cm3), high particle density (2.81 g/cm3, 2.94 g/cm3, 3.16 g/cm3), and high porosity (49.64%, 57.22%, 57.03%). In conclusion, it was observed that erection of high-rise building is not advisable within the study area since the results showed that the overburden (depth to basement) is generally thick mostly greater than 15 m. Also revealed from the microstudy of soil properties is that foundation stability should be properly managed, since a porous soil does not accommodate engineering activities except when an artificial basement is put in place

The Pattern of Soil Transmitted Nematode Infections in Primary School Children of the Kumba Health District; South-West Cameroon

Biomedical studies accompanied by a questionnaire survey were carried out on 803 pupils of classes 3; 5 an 7 attending 17 primary schools in the Kumba Health District to determine the prevalence of soil - transmitted nematodes and infection-associated morbidity. The prevalence rate of worms in the sample population from the questionnaire survey was 42.3which correlates closely with 44.2recorded for abdominal pains. The biomedical studies gave a worm infection rate of 67.1. Ascaris lumbricoides; Trichuris trichiura and hookworm had prevalence rates of 54.9; 33.8and 20.3respectively. Mixed infections were recorded in 34.4of the sample population compared to 32.8for single infections. Hookworm infection rate was significantly higher in the rural areas than in the urban areas (27.5versus 14.8; P 0.001). A similar trend was observed for T. trichiura (38.4versus 30.3; P 0.02). Prevalence of A. lumbricoides was similar in the urban and rural areas (56.5versus 52.6). Males carried heavier burdens of Ascaris and Trichuris than females. The worm burden for all 3 species decreased as children moved to higher classes and with age after the first decade of life. There was a significant correlation between the intensities of infection of A. lumbricoides and T. trichiura