Chlamydia and Vaginitis in Sexually Active Females: Classical Identification Methods for Effective Control

Laboratory diagnosis of Chlamydia and vaginitis in sexually active females has been limited by unavailability of a sequential method/rapid technique for simple diagnosis. Six hundred (600) adult females from hotel/brothel; Sexually Transmitted Infections (STIs) Clinic; Obstetrics/Gynaecology Clinic; Family Planning Clinic and Healthy controls were investigated for Chlamydia; Candida; trichomoniasis and bacterial vaginosis (BV). This was done using microscopy: wet mount; stained vaginal secretion and stained smear after culture. Results showed that there were 72infections in the female groups. The brothel and STI group had infection in the range (70-86). Chlamydial infection was highest in the STI group while Candida infection was highest in the healthy (control) females. Bacterial vaginosis was distributed in all groups. As p-value increased; f-value increased indicating constant co-infection of Candida and BV in Chlamydia positive females. Microscopy by direct detection from sample and stained smear after culture were in the range: 56-86. Direct microscopy for BV was 78.5and stained smear after culture; 57.1. Sensitivity and specificity of the techniques showed that detection of Chlamydia was less sensitive by direct microscopy of sample but sensitivity and specificity of stained smear after culture were high. Immunoassay (32.2) was also less sensitive. Sensitivity and specificity of wet mount microscopy for Candida; Trichomoniasis and BV were in the range 62.5 - 80and 62.5-97.8respectively. Wet mount has high sensitivity and specificity for detecting agents of vaginitis and may be useful for routine use and for diagnosis where disease is absent; thus; making identification more cost effective

Prevalence of Candida Albicans in Symptomatic Vulvovaginitis in Port Harcourt; Rivers State; Nigeria

The prevalence of Candida albicans in symptomatic vaginitis in female patients aged 15 - 30 years attending the University of Port Harcourt Teaching Hospital (UPTH) Clinics was investigated. A total of 120 High vaginal swab (HVS) samples were collected and examined by direct microscopy and culture. The investigation revealed that prevalence of vaginal candidiasis among these patients was 50. Candida albicans accounted for 38.3while other species of Candida accounted for 11.7. Susceptibility of C. albicans to fluconazole was examined using well-in-agar diffusion technique and 89.1of C. albicans colonies were found to be susceptible to the antifungal azole. Microscopic examination also revealed that both bacteria and Trichomonas vaginalis cause vaginitis

Vaginose bacterienne chez la femme enceinte a Cotonou (Benin)

L'etude de la vaginose bacterienne chez la femme enceinte presentant des leucorrhees; a la Maternite de ZOGBO a Cotonou (Benin) permet d'en apprecier la frequence (20;74 pour cent). Cette affection frappe les gestantes jeunes (15-29 ans); celibataires. La presence de cellules-indicatrices; un test a la potasse positif sont les facteurs predictifs d'une vaginite a Gardnerella vaginilis chez les consultantes pour leucorrhee. Les auteurs recommandent la recherche systematique de ces elements dans le cadre de l'examen bacteriologique des exsudats vaginaux en milieu tropical; ou les cultures sont generalement difficiles a realiser