Detection of the janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
Afr. j. lab. med. (Online)
; 7(1): 1-4, 2018. ilus
Article
in English
| AIM
| ID: biblio-1257318
Responsible library:
CG1.1
ABSTRACT
The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories.Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation
Full text:
Available
Index:
AIM (Africa)
Main subject:
South Africa
/
Nucleic Acids
/
Real-Time Polymerase Chain Reaction
/
Myeloproliferative Disorders
Type of study:
Diagnostic study
Country/Region as subject:
Africa
Language:
English
Journal:
Afr. j. lab. med. (Online)
Year:
2018
Type:
Article
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