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J. appl. oral sci ; 25(6): 631-640, Nov.-Dec. 2017. tab, graf
Article in English | LILACS, BBO | ID: biblio-893662

ABSTRACT

Abstract Objectives: The primary purpose of this study was to examine the effects of triethylene glycol dimethacrylate (TEGDMA) on odontoclastic differentiation in the dental pulp tissue. Material and Methods: The effects of different TEGDMA dosages on the odontoclastic differentiation capability of dental pulp cells were analyzed in vitro using the following methodologies: i) flow cytometry and tartrate-resistant acid phosphatase (TRAP) staining; ii) apoptotic effects using Annexin V staining; iii) mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor (NF)-kB ligand (RANKL) genes by quantitative Real-time PCR (qRT-PCR); and iv) OPG and RANKL protein expression by enzyme-linked immunosorbent assay (ELISA). Results: TEGDMA caused relatively less odontoclastic differentiation in comparison with the control group; however, odontoclastic differentiation augmented with increasing doses of TEGDMA (p<0.05). The mRNA and protein expression of OPG was lower in TEGDMA treated pulp cells than in the control group (p<0.05). While the mRNA expression of RANKL remained unchanged compared to the control group (p>0.05), its protein expression was higher than the control group (p<0.05). In addition, TEGDMA increased the apoptosis of dental pulp cells dose dependently. Conclusions: TEGDMA reduced the odontoclastic differentiation ability of human dental pulp cells. However, odontoclastic differentiation ratios increased proportionally with the increasing dose of TEGDMA.


Subject(s)
Humans , Polyethylene Glycols/pharmacology , Polymethacrylic Acids/pharmacology , Cell Differentiation/drug effects , Dental Pulp/drug effects , Tartrate-Resistant Acid Phosphatase/drug effects , Enzyme-Linked Immunosorbent Assay , Lipopolysaccharide Receptors/metabolism , Dental Pulp/cytology , RANK Ligand/metabolism , Real-Time Polymerase Chain Reaction , Flow Cytometry
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