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Wistar nonlinear rats weighing 170-220g. Rats were divided 5 groups, including control group, group-1, group-2, group -3 and reference group. Dried thistle extract and raw bovine testicle were contained by 1:1, 1:2 and 2:1 ratio. Each 0.1g ratio was dissolved in 20 ml distilled water and administered 2 times per day. Blood sampling was done for each rat after 3, 7, 14, 21, 28 and 35 days. Their testosterone level was measured by ELISA Kit. Results: The results indicated that free serum testosterone level in male rats increase and decrease in 7 days frequency. All tested groups showed gradual increase in the level of free serum testosterone when compared to that of corresponding control (p<0.05). Statistical comparison of all groups revealed that the maximum level was found in group 1. Moreover, group 3 was showed gradually increase in level of free serum testosterone, irrelative with period of decrease testosterone level. Conclusion: According to our results and previous study, it is suggested that preparation with Tribulus terrestris L. extract could be used in the androgen deficiency and erectile dysfunctions. Keywords: Tribulus Terrestris L, Free testosterone, Dihydrotestosterone, Protodioscin
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Introduction In recent years we have observed that there are been more and more studies and increased reg- ulatory action regarding animal, plant and mineral-based raw materials, drugs, biological prod- ucts, groceries and food products.Therefore, dehydrated cow bile liver hydrolisate appears less harm- ful for the human body and may minimize damage to liver cells, have regenerative and healing properties, and may support the healing / recovery process process. It is important to find and apply preparations that work against acute inflammation of the liver protein, fat and carbohydrate me- tabolism. Pharmacological research was undertaken with the performance of a histomorphological assessment with reference to the hydrolisate liver, dehydrated cow bile, silymarin 3 with a composi- tion containing “Sillichol”, determining how it seriously affects the inflammation of the liver operation. Goal: To determine the presence of the liver tissue morphology with reference tothe investigational / experimental new drug “Sillichol”. Material and Methods: Male Wistar rats, specified as pathogen-free, weighing 200-250 g, were obtained from the Vivarium of the Department of Pharmacology, Drug Research Institute, and were used for the chronic CCL -induced liver injury model in all experiments. Eighteen rats were di-vided into three groups (with each group consisting of 6 rats).The rats were sacrificed at the end of the 14 days of the investigation, and the livers were im- mediately removed (Methods R.Virchow). Liver slices were made from a part of the left and cen- tral lobes, and immediately fixed in 10% buffered formalin phosphate solution, embedded in par- affin, and cut into 5μm sections. Subsequently, the sections were stained with hematoxylin and eosin (H&E) and observed under a microscope to evaluate histopathological changes (20x20). Result: Liver tissue sections of the rats were stained with H&E. The histopathological assessment in the livers was performed for all groups. Rats in the negative healthy group exhibited normal, well- defined histological structures, without any signs of vascular or inflammatory changes: no cavita- tions, necrosis or fibrosis were found in normal control sections. The histopathological analysis of the livers revealed signs of toxicity after administration of CCl .This toxicity was significant in comparison with the control group and cavitations, fibrosis in board ar- eas, mild vascular congestion and moderate inflammatory changes with congested sinusoids, nu- clear changes, and centrilobular necrosis. Sinusoids spaces were flooded with inflammatory cells. The Sillichol-treated animals of the experimental group showed a complete reversal of toxic ef- fects in the liver cells; no necrosis was seen. The central vein and portal triads appear normal and show an increase of Kupffer cells. Some of the hepatocytes indicated binucleation, suggesting re- generative activity with feathery degeneration of hepatocytes.The Carsil-treated animals of the standard group: The histology of the liver sections in rats adminis- tered with Carsil indicated significant improvement with less damage of liver tissue, as indicated by a reduced level of necrosis, narrow fibrotic septae, fat storing cells, Kupffer cells, and narrow cavita- tion. Conclusions We found that the “Sillichol” biological active product treatment reduced hepatic necrosis and fibro-cal active product improved the regeneration process of liver cells, helped to normalize cell struc- ture, and had an effect on the anti-inflammatory action in damaged liver cells. Keywords: Histology, Carbon tetrachloride, Liver damage, Silichol, Liver cell
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"Introduction A chromatographer always looks forward to getting perfect shaped peaks for each and every analysis but in reality peaks get distorted due to numerous reasons. Distortions are frustrating but if proper corrective steps are taken peak shape distortions can be avoided. Peak purity can be established by taking the ratio of two wavelengths across the peaks of running samples or standard. May, if the peak is pure, then the ratio of wavelengths should be constant across the peak. Otherwise, the peak is impure, then the ratio between two wavelengths will change across the peak as the spectral differences caused by interfering peak change the signal ratio."
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Introduction: Constipation is not often regarded as a major therapeutic issue, but the use and abuse of laxatives by older people is highly prevalent. It is important for clinical to have an understanding of constipation to people, and of ways to improve laxative use. The laxatives most appropriate for older people include stimulants such as senna, bulking agents. Cassia acutifolia L and Rheum undalatum L have been used as a laxative drug in Asian traditional medicine for a long time. The pods and leaves contain antraquinone aglycons and antraquinone glycosides which are laxative form while rhein is a major component. The anti constipation ability of combinations of plant preparations consisting of Cassia acutifolia, Rheum Undalatum, Terminilia Chebula Retz, and soda were studied. Anti constipation drug set for LD50 samples of. The pharmacological experiments were done on 30 rat of wistar species weighing 200 -250 grams and 30 rabbits of Shinshilla species weighing 2000-2200 grams, 60 mouse of Balb/c species weighing 20-32 grams. The combinations of plant were given to experimental animals orally with the dose of convenient. The experimental results indicate that sample 1 compared to other samples dungy moisture to add the most during pathological induced anti constipation. Goal: The aim of study is determination anti constipation activity in plant preparations. Material and methods: Rheum undalatum L was collected from Medicinal botanical garden of Drug Research Institute in September of 2011. Cassia acutifolia, Terminilia Chebula and Soda were to be out from Monos food LLC in August of 2013. Maceration was chosen by suitable extraction method and optimal extragent was ethanol. Antraquinone was determined in evaporated extract of Cassia acutifolia, Rheum undalatum using the method by Russian Pharmacopeia. The pharmacological experiments were done on 30 rat of wistar species weighing 200 -250 grams and 30 rabbits of shinshilla species weighing 2000 -2200 grams, 60 mouse of Balb/c specie weighing 20 -32 grams. The combinations of plant were given to experimental animals orally with the dose of convenient. The experimental results indicate that sample 1 compared to other samples dungy moisture to add the most during pathological induced anti constipation. Result: Liquid extracts were obtained from Cassia acutifolia L, Rheum undalatum L with maceration method. Liquid extracts were evaporated on Vacuum rotor. Sample 1 was have anti constipation activity Keywords: Cassia acutifolia L, Rheum undalatum L, Terminilia Chebula Retz, salt, rabbits, rats, mice, and Constipation
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Introduction: This plants distributed about 2500 species in worldwide and 69 species of those grow in Mongolia. In case of medicinal application of this plant is commonly used traditional medicine in European and Asian. For example, the different preparations of Astragalus mongholicus root, the most commonly used Astragalus species in Chinese medicine. Other Astragalus species which are being investigated for their anti-cancer properties are the Turkish species, and include Astragalus brachypterus, Astragalus cephalotes, Astragalus microcephalus and Astragalus trojanus. Nowdays, this plant source from nature is decreased and areal square de-escalated in Mongolia. Goals: Our main goal was conserves domesticates and improves plant genotypes of local wild medicinal specie and provides raw plant material for scientific testing from the cultivation site without depleting wild sources. Including that main goal we put a purpose of the make comparative study of vegetative organ’s anatomy morphological study on wild and planted type. Materials and Methods: The wild Astragalus mongolicus bunge was collected from Bayanchandmani sum, for Tuv province. Cropped plant was grown medicinal botanical garden of Drug research institute, Monos. Anatomy structure of vegetative organs was identified by plant anatomic method. Result: The article presents the data on structure of vegetative organs of cultivated Astragalus mongolicus Bunge and compared with wild type. Comparative studies were made on the leaf, root, stem and flower and the anatomical structure of vegetative organs of Astragalus mongolicus Bunge and wild type, which were the similar plants on botany and had the great value of medicine. The results indicated that the anatomical structures of their vegetative organs were basically the same, but there are some obvious differences on leaf and stem it depended from geographical influences. Planted plant in particular could alter their environment by affecting light and water avialablity, soil nutrient availability due to uptake. Discussion: Tserenkhand.G was studied Astragalus mongolicus root anatomy in 2003. Our root anatomy study was same to Tserenkhand.G. Conclusion: The results from this study, we are concluded that, the cultivated Astragalus mongolicus Bunge is greatly cultivated in our Botanical garden. Key words: cultivated, anatomy, epidermis
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BACKGROUND: Fourteen species of the genus Iris L is grown in Mongolia. Iris L has been in traditional Mongolia medicine for treatment of urinary and kidney disease. We investigated three species of Iris L such as Iris Bunge /Iris Bungei Maxim/, Iris Tigrida /Iris Tigrida Bge / and Iris lactea /Iris Lactea Pall/ which contains rich amount of biological active substances[1,2]. Urine was collected at tubes during 4 hours, since given experimental and standard preparations orally and compared with control group. As experimental results, the urine levels of treatments groups increased 1.13 fold (Iris Bunge), 1.8 fold (Iris Tigrida), 1.04 fold (Iris Lacteal) and 1.15 fold (Standard or Nefromon) compared with control groups. These results indicated the Iris Tigrida has shown diuretic effect more than other species of Iris L.\Z TOOLS AND METHODS: Adult Balb/c mice, each in the weight range of 20-28g, were obtained from the Vivarium, Drug research institute. The animals divided into five groups of four animals each and kept in cages and housed under standard conditions of temperature, humidity and dark light cycle (12h-12h). Diuretic activity was determined by the following methods of Trendelenburg et al (1980). Before experiment, all the animals received priming dose of 0.9% sodium chloride solution (0.3 ml/20g) and the treated groups were given 0.1ml/20g extracts of Iris L. The standard groups were given 0.1 ml/20g of “Nefromon. Immediately after respective treatments the animals were placed in metabolic cages and urine was collected in a measuring cylinder till 4 hours. RESULTS AND CONCLUSION: Results are shown in the ethanol extract of the whole plant of Iris Tigrida Bge at a dose of 0.1ml/20g observed diuresis during the 4 hours of the test (Tigrida Bge 1.0±0.37 ml versus control 0.55±009 ml, whereas in case of standard Nefromon the volume was found to be 0.63±0.08 ml, p<0.05). As experimental Iris L, results, urine levels of treatments groups increased 1.04 fold (Iris Bunge), 1.8 fold (Iris Tigrida), 1.05 fold (Iris Lacteal) and 1.15 fold (Standard or Nefromon) compared with control groups. These result, indicated the Iris Tigrida has shown diuretic effect more than other species of Iris L. These results indicated the Iris Tigrida has shown diuretic effect more than other species of Iris L. KEY WORDS: Diuretic, medicinal plants, Trendelenburg methods, flavonoids