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Introduction: Constipation is highly prevalent, often chronic gastrointestinal disorder that affects adults. The treatment with classic drugs did not cut, in one hand with the inadequate relief of bloating and other symptoms, and with the luck of efficacy in relieving constipation. Therefore, the search for novel safe laxative drugs seems, inevitable. Rheum undulatum L. was traditionally used in constipation, thus we have attempted to evaluate the laxative effect of Rheum undulatum L. Purpose: The laxative effect of Rheum undulatum L. was evaluated against loperamide induced constipated rats. Methodology: Fifteen male normal rats were used in this study. Fifteen male constipated wistar albino rats weighing 180-250 g were also used for the study and randomized into three groups (n=5) in each of the experiments. Constipated control group rats oral administrated distilled water. Constipated rats (treatment groups) were treated with 4.1 mg/kg dose body weight /day of the preparation for one day and also Laxing a standard drug was used for the reference group. The fecal weight, the fecal humidity laxative activity were monitored in experimental rats.Results: Constipation was successfully induced in the rats by loperamide as seen in the elevated fecal properties compared to the control rats. The Rheum undulatum L. compounds preparation administered orally produced significant laxative activity and reduced loperamide induced constipation in dose dependent manner as seen in the increase of fecal output. The same doses of the Rheum undulatum L. compounds preparation produced a significant increase (P<0.05) fecal weight, the faeces humidity. The effect of the compounds preparation compares favourably well with Laxing, a standard laxative drug. Conclusion: The results of this study justify the use of Rheum undulatum L. compounds preparation as a laxative in traditional medicine. The produced significantly increase in fecal output of rats and the stimulation of gastrointestinal motility. Keywords: Laxative, gastro intestinal motility, loperamide, constipated
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IntroductionIn recent years we have observed that there are been more and more studies and increased reg- ulatory action regarding animal, plant and mineral-based raw materials, drugs, biological prod- ucts, groceries and food products.Therefore, dehydrated cow bile liver hydrolisate appears less harm- ful for the human body and may minimize damage to liver cells, have regenerative and healing properties, and may support the healing / recovery process process. It is important to find and apply preparations that work against acute inflammation of the liver protein, fat and carbohydrate me- tabolism. Pharmacological research was undertaken with the performance of a histomorphological assessment with reference to the hydrolisate liver, dehydrated cow bile, silymarin 3 with a composi- tion containing “Sillichol”, determining how it seriously affects the inflammation of the liver operation.Goal: To determine the presence of the liver tissue morphology with reference tothe investigational/ experimental new drug “Sillichol”.Material and Methods: Male Wistar rats, specified as pathogen-free, weighing 200-250 g, wereobtained from the Vivarium of the Department of Pharmacology, Drug Research Institute, andwere used for the chronic CCL -induced liver injury model in all experiments. Eighteen rats were di-vided into three groups (with each group consisting of 6 rats).The rats were sacrificed at the end of the 14 days of the investigation, and the livers were im- mediately removed (Methods R.Virchow). Liver slices were made from a part of the left and cen- tral lobes, and immediately fixed in 10% buffered formalin phosphate solution, embedded in par- affin, and cut into 5μm sections. Subsequently, the sections were stained with hematoxylin and eosin (H&E) and observed under a microscope to evaluate histopathological changes (20x20).Result: Liver tissue sections of the rats were stained with H&E. The histopathological assessment in the livers was performed for all groups. Rats in the negative healthy group exhibited normal, well- defined histological structures, without any signs of vascular or inflammatory changes: no cavita- tions, necrosis or fibrosis were found in normal control sections.The histopathological analysis of the livers revealed signs of toxicity after administration of CCl .This toxicity was significant in comparison with the control group and cavitations, fibrosis in board ar-eas, mild vascular congestion and moderate inflammatory changes with congested sinusoids, nu- clear changes, and centrilobular necrosis. Sinusoids spaces were flooded with inflammatory cells.The Sillichol-treated animals of the experimental group showed a complete reversal of toxic ef- fects in the liver cells; no necrosis was seen. The central vein and portal triads appear normal and show an increase of Kupffer cells. Some of the hepatocytes indicated binucleation, suggesting re- generative activity with feathery degeneration of hepatocytes.The Carsil-treated animals of the standard group: The histology of the liver sections in rats adminis-tered with Carsil indicated significant improvement with less damage of liver tissue, as indicated by a reduced level of necrosis, narrow fibrotic septae, fat storing cells, Kupffer cells, and narrow cavita- tion.ConclusionsWe found that the “Sillichol” biological active product treatment reduced hepatic necrosis and fibro-cal active product improved the regeneration process of liver cells, helped to normalize cell struc-ture, and had an effect on the anti-inflammatory action in damaged liver cells.Keywords: Histology, Carbon tetrachloride, Liver damage, Silichol, Liver cell
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Introduction:The present study shows the processing of the pharmacopoeia article for the preparation of Di- clofenac sodium extended release matrix tablet formulated from alternative characteristic poly- mer. The matrix tablets were prepared according to traditional method of the wet granula- tion for tablet form medicine.Method: A standardization study made use of several international pharmacopoeias, such as the Mongolian National Pharmacopoeia (MNP 2011), United States Pharmacopoe- ia (USP 32), Chinese Pharmacopoeia (CP 2010) and U.S Department of F.D.A for the sub- stance’s requirements.Result: The results from this study showed a completely processed manufacturing pharma- copoeia issue of the Diclomon 100 mg retard tablet and its technological and standardized re- quirements.
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Abstract:In the recent years, The pharmaceutical manufacturers interest to natural medicines and biological active preparations from animal, plant and mineral raw materials. According to some sources, Plantago majus contains polysaccharides and aucubin. Some researchers investigated aucubin that is anti inflammation and antimicrobial activity. Many anti cough formulations that are contains Plantago majus sold in pharmaceutical market. For example: Herbion, Pectoral manufactured by KRKA, Naturwaen and Mepha, respectively.The aim of this study determination of aucubin and biological active substances in Plantago majus that is growth commonly in Mongolia.The result of this study, Plantago majus leaves are estabilished polysaccharide 4.66%. Leaves of Plantago majus has been extracted by water(1:5), water (1:10), 30 % sorbitol and 20% ethanol, then determination aucubin and polysaccharide. The results, polysaccharide was 4.12%, 3.46%, 0.336% and 2.65%, respectively. Aucubin was 2.88%, 3.44%, not determined 30% sorbitol extract and 1.88%, respectively.
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Introduction: Throughout the world, there are a number of plants that have been identified with immune boosting ability and the following plants Salsola laricifolia Turcz, Inula helenium that have been proven to support the immune system and grow in Mongolia were selected for a phytochemical study and prepared technology of dried powder.Goal: To develop dry extract technology with immunity stimulating action from natural plant sources.Material and methods: A biological active substance coumarin and flavonoid determination byspectrophotometer.Result: Total coumarin in raw material of Salsola laricifolia was 2.9±0.03%, total flavonoid amount was 1.66±0.021%, total polysaccharide was 3.83±0.025%, humidity was 5.7±0.01%, extractive substance amount was 20.63±0.91% ( p≤0.05). Total polysaccharide in raw material of Inula helenium was 20.78±0.03%, humidity was 6.03±0.03%, extractive substances amount was 21.76±0.23.Salsola laricifolia’s polysaccharide content was the highest or 0.19±0.031%, when extracted with 30% ethanol the flavonoid content was 0.36% when 25%, 30% ethanol was used as the extragent, 30% ethanol is determined to be an appropriate extragent. Inula helenium’s water extract contained 3.75±0.05% polysaccharide, 0.43±0.005% flavonoid. 50% alcohol extract had a 3.20±0.01% polysaccharide, flavonoid content of 0.25±0.01% and water extragent is determined to be proper extragent in future research.The scheme of dry extract preparation technology was developed and determined quantitative indication. Total polysaccharide which was active substance of Salsola laricifolia’s 30% ethanol and microcrystal cellulose under 15:1 version was highest in 0.46±0.011% and total polysaccharide which was active substance of Inula helenium water extract and microcrystal cellulose under 10:1 version was highest in 3.46±0.021% that`s why, it is suitable to dry extracting Salsola laricifolia’s 30% ethanol and microcrystal cellulose under 15:1 version, Inula helenium water extract and microcrystal cellulose under 10:1 version.Key word: Inula helenium, Salsola laricifolia, immunity support, dry extract
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Abstract: In this article, we report a study of assay of sesquiterpene lactones (alantolactone, isoalantolactone) in plant extraction derived by ultrasound-assisted extraction, оrthogonal test design and reflux extraction from medicinal plant’s composition (Salsola laricifolia turcz.e litv+Inula helenium). High-performance liquid chromatography (HPLC) method was used for determination of the contents of alantolactone and isoalantolactone in the investigated extracts. The result shown that the amount of alantolactone was 0.64±0.03%, and isoalantolactone 0.59±0.01% in the plant extraction derived by reflux condensation extraction. Key words: Alantolactone, Isoalantolactone, HPLC, Salsola laricifolia turcz.ex litv, Inula helenium, Reflux method, Ultrasound- assisted extraction.
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Background: Rhodiola rosea L. root has used in Western and Eastern countries medical practice widelyfor medicine raw material purpose. Roseroot grows in the high mountains very rarity. In Mongolia thisplant distribute in Khuvsgul, Khentii, Khangai, Uliastai, Khankhukhii, Mongol Daguur (Western), Khovd,(Kharkhiraa), Mongol Altai, Ikh, Baga Bogd (from west longituide 88000 to east side until 110000, and fromnorth latitude 45000), and Dundad Khalkh (Bichigt Dulaan Khad) etc.Dig up the root to use it therefore the ground does not reconstruct or grow by itself. Less thrift, no reservesto use and rare greenery.Goals: The aim of this study was protects its gene bank and preserve the natural resource to doing practiceof rehabilitation therefore chance reserve and could supply raw materials for producing medicine as many aspossible in factory.Materials and Methods: The source material of the Rhodiola rosea L was collected from the Khankh sumof Khuvsgul province.Plantation procedure was done according to planting method of Erdenejav etc (1986), the analysis of kineticsof plant was studied method Ts.Jamsran (1984), was availability of naturalize planting was studied methodof R.A.Karpisinova (1985).The seeds were seeded in 40-45 cm between the row, 20-25 cm between the plants and 3.5-4.5 cm depthin the soil.Results: In the first year for naturalizing field plantlet the Rhodiola roea L. roots wild intergowth 85-90%,during the first growing stage it was regular and the hidden browse set for 1 greenery 4-6 pieces in the rootsmouth and end of the September it becomes visible in the ground.For the 2 year old Rhodiola rosea L. hidden browse during Y/10 stage the first young browse is coming upand greenery growing process is good, it’s height is 30-45 cm and in the main grasp graft’s on the top formedflower stuff, becoming fruitage and growing process become active. In the roots mouth restructuring browseis developed.The 3 year old Rhodiola rosea L. regrowth browse is good, root system become strong and developed alsoflowered and starting to give its seed. Roots system become strong and greenery grown 50-55 cm high.Rhodiola rosea L. is resistible to the cold and looking at the laboratory intergrowth seed picked from the 3 yearold greenery is was 42-48%. Rhodiola rosea L. which we are planting can be use planted in the MongolianSteppe zone. Planted 3 years old of biology active cells of root for plant’s salidroside content is 0.25%.Key words: raw material, regrowth