ABSTRACT
This study has been undertaken at Tissue Culture Lab., El-Zoharya Bot. Garden, Ministry of Agric., Egypt, and Fac. of Agric. Cairo Univ., during the period 2001-2004 to study the effect of sterilization treatments on percentage of free of contamination explants and the effect of benzyladenine [BA], Kinetin [Kin] and Thidiazuron [TDZ] and different combinations of benzyladenine [BA] and Kinetin [Kin] on shooting behavior and chemical composition of Ruscus hypoglossum L. The best treatment which can be recommended to obtain free of contamination explants was 50 or 60% chlorox with 0.4% mercuric chloride [M.C.].The addition of BA at 1.5 mg/l in MS medium produced the highest number of shoots/explant. Increasing the number of subcultures significantly increased the number of shootlets, shootlet length, and number of leaves per explant. Using MS medium supplemented with 6.0 mg/l Kin gave the longest shoots, and the highest number of leaves. Using MS supplemented with TDZ at 4.5 mg/l produced the highest amounts of chlorophyll-A, and carotenoids, while TDZ at 6.0 mg/l produced the highest amount of chlorophyll-B. The highest content of indoles was produced by using MS medium supplemented with TDZ at 3.0 mg/l. Using 1.5 mg/L BA + 2.0 mg/l Kin produced the highest number of shootlets per explant, shoot length and number of leaves. Using MS medium free hormones [control] increased the amount of chlorophyll-A to the highest value, but the highest amount of chlorophyll-B and carotenoids was produced by using MS medium supplemented with 1.5 mg/l BA + 2.0 mg/l Kin. The amount of indoles was increased to the highest amount by using MS 7nedium supplemented with 1.5 mg/l BA + 2.0 mg/l Kin. While total soluble phenols reached to the highest amount by using MS medium supplemented with 6.0 mg/l BA + 2.0 mg/l Kin
ABSTRACT
This study was carried out at Tissue Culture Lab., El-Zoharya Bot. Garden, Ministry of Agric., Egypt, and Fac. of Agric. Cairo Univ., during the period 2001 -2004 to study the effect of MS salts strength, sucrose, IBA and activated charcoal on shooting behavior, chemical composition of shootlets and rooting of Ruscus hypoglossum. The effect of some growing media during adaptation stage was also studied. Using MS medium at full salt strength produced the highest number of shoots. Increasing the number of subcultures significantly increased the number of shootlets, shootlet length, and leaves per shootlet. Using MS medium at ½ and ¼ strength increased the shootlet contents of chlorophyll-A and carotenoids to the highest values. Culturing Ruscus explants on 1/4 MS strength medium produced the greatest amount of indoles. Using 30 and 40 gm sucrose/l gave the highest number of shoots and leaves per shoot. While the highest shootlet length was produced by using 50 gm sucrose/l. Increasing the number of subcultures significantly increased the number of shoots, shootlet length, and number of leaves per shootlet. Using 10 and 30 gm sucrose /l produced the highest amount of chlorophyll-A. Using 10, 20, 30 and 40 gm sucrose /l produced the highest amount of total indoles and total soluble phenols.MS. medium at ½ strength supplemented with IBA at 2.0 mg/l was the most effective treatment in increasing the number of roots / shootlet. The highest number of roots was recorded with 50 gm/7 sucrose with activated charcoal. The longest root and shoot were recorded with 55 gm/l sucrose, while the highest number of leaves was recorded with 40 gm/7 sucrose. Adding activated charcoal to sucrose concentrations was the best treatment to obtain the longest root and shootlet, while the highest number of leaves per shootlet was shown with treatments without activated charcoal. During adaptaion the tallest plants and the greatest number of leaves were observed when using the peat moss as a growing medium, where the survival percentage was 100 %