Relationship between DNA polymorphisms at the BCL11A and HBS1L-MYB loci in beta-Thalassemia patients with increased fetal hemoglobin levels

High fetal hemoglobin [HbF] levels have a major impact on the hemoglobin disorders, i.e. beta -Thalassemia. Increased HbF production ameliorates the disease severity. Three loci-HBS1L-MYB intergenic region on chromosome 6q23, BCL11A on chromosome 2pl6, and the chi-globin gene on chromosome 11 account for up to 50% of the variations in HbF levels in patients with sickle cell anemia, thalassemia and healthy adults. In the present study, we evaluated the relationship between some polymorphisms on HBS1L-MYB BCL11A loci and increased HbF levels in thalassemia patients and normal subjects. In this case-control study, three common polymorphisms among 50 beta-thalassemia patients with increased HbF and 47 healthy individuals with normal HbF by using PCR-RFLP were genotyped: rs4895441, rsl 1886868, and rs28384513. Enzymatic digestion was performed by Rsal, MboII, and BstXI, respectively. Correlations with high levels of HbF were performed with a Chi-square test by using SPSS 16 and SNP analyzer2. Mutant allelic frequencies were 0.245, 0.521 and 0.309 in healthy and 0.3, 0.52 and 0.28 in patient for rs4895441, rsl 1886868 and rs28384513, respectively. Significant relationship was not observed among three polymorphisms studied in healthy volunteers and beta-Thalassemia major patients with increased HbF levels and P-value allelic and genotypic was higher than 0.05 at three SNPs. In spite of previous reports, evaluation of polymorphisms at the BCL11A and HBS1L-MYB loci in this study did not show up a significant correlation with increased HbF. Other polymorphisms might have a role in increasing HbF in our population

[ incidence of change in canal centring of the root canal following canal preparation with hand and rotary reciprocal techniques by stainless steel and nickel titanium files]

Cleaning and shaping of the root canal system is an important objective of root canal therapy. Many automatic devices have been produced to simplify the preparation, and to decrease instrumentation time. One of these automatic devices with the general name of Endogripper is the new reciprocal handpiece called TEP-E10R. The purpose of this study was to compare hand and rotary reciprocal techniques in incidence of change in canal centring of the root canal. In this invitro study, 60 extracted mesial roots [120 canals] of human mandibular first and second molars were selected. The mesial roots were mounted in a metallic muffle. Sixty specimens were divided into four groups, [each group consisted of 30 canals]. Each root was sectioned in two horizontal directions, the first cut was made five mm from the apical and the latter cut was made in the middle area of the root. The sections were photographed before instrumentation and then were reassembled in the metallic muffle and instrumented. In group one the preparations were made by NiTi files with hand instrumentation. In group two the preparations were made by stainless steel files with hand instrumentation, while in group three the preparations were made by Ni Ti files with use of TEP-E10R reciprocal handpiece, and in group four the preparations were made by stainless steel files and use of the same handpiece as in group three. After instrumentation, the metallic muffle was disassembled and the sections were photographed for the second time after preparation. The pre and post instrumentation photographs were superimposed using Adobe Photoshop and the canal center displacement in each section was measured. The collected data were analyzed with ANOVA and Tukey statistical tests. All specimens in the four groups showed canal center displacement. Group two had the most mean of canal center displacement in coronal sections which was significantly different from group one [p=0.008]. In the middle sections, the highest mean of canal center 2 displacement was seen in group four and it was significantly different from group one [p=0.001]. In the apical sections, group two had the highest mean of canal center displacement which was significantly different from other three groups [p<0.001]. Canal preparation with reciprocal handpiece [TEP-E10R] was similar to hand preparation technique with Ni Ti files in shaping of the root canal system

[Comparison of root canal transportation during canal preparation with hand instrumentation and reciprocal automatic device]

Cleaning and shaping of the root canal system is an important objective of root canal therapy. Many automatic devices have been produced to simplify the preparation and to decrease instrumentation time. One of these automatic devices is the new reciprocal handpiece [TEP-E10R] known as general name "Endogripper". The purpose of this study was to compare canal transportation with hand instrumentation technique and reciprocal instrumentation technique with TEP-E10R handpiece. In this invitro study 60 human mandibular first and second molars were selected and divided into four groups. Radiographs with initial file inserted to working length were taken in all specimens with constant conditions. In group one, the preparations were made by Ni-Ti files and hand instrumentation technique. In group two, the preparation were made by stainless steel files and the same technique in group one, in group three preparation were made by Ni-Ti files and TEP-E10R reciprocal handpiece and in group four, the preparation were made by stainless steel files and the same handpiece in group three. Instrumentation technique in all four groups was crown down preparation. After finishing of the instrumentation in latter four groups, additional radiographs were taken while the master apical files [MAF] were inserted in the canals with the same constant conditions recorded for intial file radiographs. The MAF radiographs were compared with initial file radiographs for canal trans portation. The amount of dentin removal was also calculated by measuring instrumented surface in the final radiographs. Data were collected by SPSS10. One way ANOVA analysis was used to compare four groups and tukey test was used to compare couple groups. All of specimens showed degrees of transportation. Group two had the most mean degree of transportation that was significantly different from group one [P=0.001]. None of the groups had statistically significant difference between amount of dentin removed from canal walls [P=0.83]. Reciprocal handpices with NiTi files had acceptable results for canal preparation