ABSTRACT
Background: intra Cytoplasmic Sperm Injection [ICSI] is a reliable method for single sperm selection and injection to a large number of infertile patients. Human oocytes are naturally surrounded by cumulus cells embedded in the intracellular matrix made primarily of hyaluronic acid [HA], which plays a role as physiological selector for Intra-Cytoplasmic Sperm Injection [PICSI]. Mature spermatozoa are able to bind to and digest HA for the best chance of reaching the oocyte and to maintain fertilization. The intact human spermatozoa are bound to immobilize HA surrounding oocyte in vitro and this leads to reduced risk of chromosomal imbalance or chromatin diseases. Selection of spermatozoa by HA before ICSI may help to optimize the outcomes of the treatment; thus, our study aimed to compare ICSI results, based on the hyaluronic acid or traditional method for sperm selection
Setting: this study conducted at International Islamic Center for Population Studies and Research, Assisted Reproduction Unit, Al-Azhar University from January to October 2015
Patients and Methods: 120 couples with male factor infertility and normal wives conducted at the Assisted Reproduction Unit in the International Islamic Center for Population Studies and Research [IICPSR], Al-Azhar University, Egypt, during the period from January to October 2015. All cases were clinically evaluated and eligible for analysis by using inclusion and exclusion criteria. All studied men subjected to ejaculated sperm prepared by traditional method and another prepared by PICSI method. ICSI performed simultaneously for different oocytes from the same woman using an ejaculated sperm by two methods from the same husband. ICSI outcomes compared to the two sequential attempts performed, respectively. Number and quality of oocytes, fertilization rates, rate of embryo cleavage and pregnancy outcomes were recorded. The collected data were tabulated and statistically analyzed
Results: the present study showed a significant increase in the incidence of fertilization rate, the percentage of embryos with top grade in teratozoospermia and thawed semen groups with PICSI technique compared to the traditional method of ICSI groups. Finally, the percentage of pregnancy rate an increase in all groups manipulated with PICSI technique and this increase was statistically significant
Conclusion: this study shed more light on the physiological sperm selection method [PICSI] which may improve fertilization and pregnancy rates compared to the traditional selected sperm in ICSI. It is concluded that PICSI technique in Assisted Reproductive Technology [ART] is one of the very important technique that improve fertilization in case of low fertilization rate after ICSI
ABSTRACT
The study aimed to determine the factors that affect nurse utilization of evidence based practices for nursing care of pregnant women with toxemia. Research questions of the study are there barriers that affecting the utilization of the evidence-based practices for pregnant women with toxemia? A descriptive study design was conducted at inpatient units and intensive care unit in [Ain Shams and Suez Canal] Maternity University Hospitals. Convenient sample was used to recruit 110 nurses' according to criteria of the study. Two tools were used for data collection. First tool: Arabic structured interviewing questionnaire sheet to assess: First part, demographic characteristics of study sample and second part, assess factors that affect nurse utilization of evidence based practices. Third part, knowledge assessment sheet to assess nurse's knowledge regarding EBP. Second tool observational check list: for assess nurse's practices regarding EBP. The duration of data collection started from October 2009 and completed by October 2010. Result of the present study revealed that, about slightly more than one third of nurses [36.4%] were has unsatisfactory knowledge about EBP ,Most of studied nurses [80%] didn't utilize EBP process, these results due some factors as lack of technological skills in find evidence [96.4%] and resistance to change are represented by [80%] between studied nurses. In addition, the present study revealed that, Positive significant correlation was found between both nurses' [Knowledge's and Practices] of EBP in relation to their qualification [highly qualified nurse], while there is negative significant correlation with their experience [< 10 years], from The present study concluded that, still there is a gap between EBP and toxemia care for pregnant women due to many barriers as lack of communication skills with technology, inability to read and analysis the EBP researches, not interested with changes or new scientific evidence, insufficient time to apply EBP in clinical area with patient. In the light of the study findings the researchers can recommend that enhance obligatory and motivation system for nursing staff to inform and apply EBP In their specialties bedside, improve awareness of nurses about the importance of application of EBP on health filed through training program for nurses, in different maternity services. In the light of the study findings the researcher can recommend that enhance obligatory and motivation system for nursing staff to inform and apply EBP. In their specialty bedside, improve awareness of nurses about the importance of application on health filed through training program for nurses, about nursing care of patient in different maternity services