Detection of Borrelia persica infection in Ornithodoros tholozani using PCR targeting rrs gene and xenodiagnosis

Relapsing fever caused by Borrelia persica, is an acute tick-borne disease which is transmitted by soft ticks of Ornithodoros tholozani to human. Value of PCR and xenodiagnosis for detection of B. persica in O. tholozani ticks was compared. Sixty-four Borrelia- free ticks were fed on infected guinea pigs and used for the experiments. For xenodiagnosis, a group of 32 ticks in subsequent blood meal were fed on sterile guinea pigs and the indication of B. persica in the animal blood was tested 5-14 days later by dark-field microscopy. For PCR, all 64 ticks were subjected to PCR against B. persica rrs gene [16S-rDNA]. Also sensitivity of PCR in terms of minimum detectable number of spirochetes as well as the effects of tick sex and post digestion was tested. PCR revealed B.persica DNA in 98.4% ticks, in which B. persica were found in 25.0% by xenodiagnosis. PCR was enough sensitive to give positive results for DNA of 1 spirochete. PCR success rates were similar for male or female ticks. Course of time did not affect the efficacy of PCR and similar results were observed for ticks of immediately fed, semior completely gravid or completely digested blood ones. Our results indicate that due to very low specificity and time consuming, xenodiagnosis is not a useful method whereas PCR method has advantages for study the Borrelia prevalence in ticks

Study on presence of borrelia persica in soft ticks in Western Iran

A molecular survey was conducted to investigate the presence of pathogenic Borrelia persica species causing the tick borne relapsing fever [TBRF] in Takistan district Qazvin Province, western Iran. A number of 1021 soft ticks were collected from 31 villages including previously reported infected and none-infected TBRF cases and individually examined for the presence of B. persica DNA by conventional PCR targeting the 16SrRNA. A total of 1021 soft ticks of three species of Ornithodouros tholozani [120: 11.75%]. O. lahorensis [461: 45.15%] and Argaspersicus [440: 43.1%] were collected and tested against Borrelia infection. Soft ticks were more prevalent [67%] in infected areas than none infected areas. The rate O. tholozani in infected areas was much greater [29 times] than none infected areas. Ninety seven percent of soft ticks in none infected areas were of O. tholozani. Sixteen [16.7%] ticks of tested [n=95] O, tholozani were infected with B. persica. Three [1.3%] out of 205 soft ticks of O. lahorensis were positive for Borrelia sp., and no infection was observed in A persicus. Taq/ RFLP analysis and sequence analysis of the positive PCR products showed the presence of B. persica. The RFLP analysis showed that the positive ticks of O. lahorensis were infected with unknown Borrelia species. This study showed that although there were no TBRF cases in Takisan. but still infected O. tholozani, the known vector of TBRF. presented in the region. Control measures needs to be fulfilled in Thakisan