ABSTRACT
Background and Objective: Wilson's disease [WD] is caused by mutation to the cooer-transporting gene ATP7B. Chelation therapy is the main protochol of treatment for patients with Wilson's disease. D-penicillamine is one of the well-known chelator agants which is used in WD treatment but it can not enter into the intracellular space.This study was done to evaluate the synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine -Dendrimer in Wilson's model cells
Methods: In this descriptive-analytic study, initially 0.01 mm polyethylene glycol [PEG] and 0.0018 mm citric acid, Dendrimer was synthesized. After purification by dialysis bag and lyophilization, lOmg dendrimer was conjugated to 3.3mg D-penicillamine. Nanoconjugated D-penicillamine-dendrimer was injected on Wilson's model cells. After incubation and centrifugation intracellular measurement of copper concentration and FTIR test were done
Results: Copper accumulation significantly reduced in the HepG2 WD cell by Nanoconjugated D-penicillamine - Dendrimer in compared to D-penicillamine [P<0.05]. Copper accumulation was determined to be 46.61, MTT assay showed no toxicological damage in HepG2 WD cell
Conclusion: Nanoconjugated D-penicillamine -Dendrimer can reduces intracellular concentration of Copper
Subject(s)
Penicillamine , Dendrimers , CopperABSTRACT
Background: study on the effects of some additives to reduce the negative effects of physiological stress in poultry
Objectives: this study was conducted to investigate the effects of the dietary synbiotic Biomin-IMBO and electrolytes-multivitamin solution in drinking water on performance of Japanese quail under physiological stress
Methods: a total of 240 one-day-old Japanese quail [Coturnix Coturnix japonica] were randomly assigned to 4 treatments with 4 replicates and 15 birds each. Experimental diets were 1- diets based on corn and soybean meal [negative control], 2- basal diets+ dexamethasone [positive control], 3- positive control+ 1gr/kg diet Biomin-IMBO[registered sign], 4- positive control+ 2 ml/li water electrolyte-multi vitamin
Results: physiological stress significantly reduced feed intake and live body weights of quails [p<0.05]. Feed consumption of stressed bird increased by adding electrolytes-multivitamin to drinking water [p<0.05]. Dietary Biomin-IMBO supplementation decreased significantly heterophil:lymphosyte ratio in blood of stressed birds [p<0.05]. The concentration of glucose in serum of positive control and water electrolyte-multivitamin group were lower than birds in negative control at stress period [p<0.05]
Conclusions: dietary Biomin-IMBO supplementation could reduce negative effect of physiological stress in Japanese quails
ABSTRACT
In recent years isolation of antibiotic resistant Staphylococcus aureus, especially methicillin resistant Staphylococcus aureus in food and food-producing animals has become more frequent. The aim of this study was to determine the prevalence of MRSAin traditional white cheese and butter presented in Tabriz by culture and PCR techniques, as well as the determination of their antibiotical susceptibility. In the present study, 250 traditional white cheese and butter samples were collected from different producers across Tabriz. Evaluation for contamination by coagulase positive Staphylococcus aureus was done using the Culturing method. The isolates were subjected to the PCR technique according to the Nuc gene in order to confirm Staphylococcus aureusand methicillin resistance to the gene [mecA] and the antibiotic resistance trend of the isolates were studied by the Disc Diffusion Agar method. The culture of the samples indicated that 26 cheese and 24 butter samples were contaminated by Coagulase Positive Staphylococcus aureus. Nineteen cheese samples and 11 butter samples were confirmed to be Staphulococcus aureus based on PCR using the Nuc primer gene. From a total number of 30 isolates containing the nuc gene, 11 had the resistant mec-Agene. In overall, from the evaluated samples 100% of the isolates demonstrated simultaneous resistance to more than 3 antibiotics. It can be stated that there was a considerable amount of Staphylococcus aureus which was resistant to a variety of antibiotics, most importantly methicillin resistant. Staphylococcus aureus in the evaluated samples
Subject(s)
Staphylococcus aureus , Methicillin Resistance , Cheese/microbiology , Prevalence , Polymerase Chain Reaction , Microbial Sensitivity TestsABSTRACT
Although coronary angiography is gold standard for diagnosis of coronary artery disease, it is nevertheless an invasive and potentially hazardous procedure. The aim of this study was to investigate the predictive value of carotid and femoral artery Intima-Media Thickness [IMT] for detection of coronary artery disease. The present study comprised 100 consecutive patients referred for coronary angiography due to symptoms of ischemic heart disease. Ultrasound assessment of common carotid and common femoral artery were performed with an ultrasound device equipped with a high-resolution transducer. IMT was measured in the common carotid and common femoral artery [10 mm proximal to the deep femoral artery origin]. There were 25 cases in each of single [S], double [D] and triple [T] vessel disease and 25 in significant left main diseases groups of patients. In regard to common carotid artery IMT was 0.78 mm in S, 0.84 mm in D, 0.97 mm in T and 1.05 mm in left main disease groups. There was a significant correlation between IMT measured in the carotid artery and severity of coronary artery disease [P = 0.0001]. With respect to common femoral artery IMT was 0.66 mm in S group, 0.73 mm in D group, 0.84 mm in T groups and 0.85 mm in patients with left main disease.. There is a significant correlation between IMT [measured in the common femoral artery] and severity of coronary artery disease [P = 0.0001]. Our results indicated that early atherosclerosis in both carotid and femoral arteries were highly predictive of coronary involvement and IMT being associated with the number of coronary vessels disorder
ABSTRACT
Several methods are available for control release of propranolol hydrochloride [PLH]. The aim of the present study was to develop a novel technique to sustain PLH release from matrices. Matrices of PLH containing sodium carboxymethylcellulose [Na CMC] and various amounts of the inorganic cations Ca[2+] and A1[3+] were prepared. Dissolution of the matrices was carried out using the USP apparatus I. Analysis of release data was performed by some model independent and dependent approaches. The release of PLH was affected by incorporation of different amounts [milliequivalents, meq] of Ca[2+] 2+ and Al[3+]. When the Ca[2+] amount increased from 0- 0.375 meq, the fraction of PLH which released within 480 min was augmented from 0.74 to 1 apparently via disintegrating effect of the cation. Al[3+] in the range 0- 0.125 meq, decreased the fractional release from 0.74 to 0.37 presumably by in situ cross- linking with polymer. Al[3+] between 0.125 and 0.5 meq enhanced the release from 0.37 to 1 possibly due to the disintegrating effect. Among model independent metrics, the mean release time [MRT] failed to represent the effect of the cations on the release but the release efficiency [RE] as well as a suggested mean release rate [MRR] correlated well with the experimental release rate. Due to the complexity of the release, the only suitable kinetic model was the Weibull distribution. The minimum and maximum Weibull release rate constants for matrices containing Al[3+] were 0.0007-0.017 1/min. The corresponding values for the matrices with Ca[2+] were 0.0029-0.0082 1/min. Through careful choice of the amount of Al[3+] in NaCMC matrices the release of PLH can be controlled at a desired rate. The best model independent approach is MRR and the most accurate model dependent method is Weibull distribution to describe the release data