ABSTRACT
Cells and embryos culture are new, important and useful methods in plant science scope. This method moved out from research to application scientific circuit. The technique of tissue culture uses the rapid propagation to produce large number of plants this cannot be actualize by classical propagation method which needs to specific time in the year and effort. Large number of scientific researchers have done that leaded know how can parts of plant differentiate and develop to organisms in artificial media. In order to, grow part of the plant. It should be capable of mitosis division where testing of used part of the plant and the growth stage of this part are important. In any case, should sterilize the used plant part before agriculture start then provide it with growth media. This requires that all equipment used in creating a cell and embryos culture must be sterilized to ensure contamination does not occur. In plant tissue culture researchers faces many problems as kind of nutrition media, high quality of sterilization that the. In addition to stimulation of embryos growth and activation of embryos to grow. This study was conducted in order to study possibility for alteration ager media to cellulose media [cement papers] for germination and growth of peas plant embryos. The mature embryos separated, sterilized then germinated in ager and cellulose media, in order to compare their growth in both media. Embryos left to grow in dark incubator at 26°C for four days. Growth parameters recorded then ail seedlings exposed to hardness stage by transferring them to the small pots. Seedlings left to grow four days under controlled conditions. Results of this study revealed that cellulose papers media is the best for embryos growth especially during the first four days [Incubation stage] where highly significant increases in total plant length; leaves formation had noted. In contrast, embryos that germinated in ager media did not showed any response in their length and weight .Treatment of embryos that grown on cellulose papers media by Gebberelic acid concentration of Ippm significantly increased their lengths; fresh weight and water content, while, treatment of embryos, that grown on ager media, with the same concentration of Gibberellic acid was useless during the first four days [Incubation stage]. After transfer the seedling to hardness stage, it had noted that seedlings grown in cellulose papers media improved their growth when they compared with seedlings that grown on ager media, also, the seedling slants to show an increase especially in the length of root. Treatment of seedlings by Gibberellic acid during the stage of hardness was not usefulness because it did not note any significant increases between controlled plants and the treated plants. The result of this study revealed to that cellulose papers can be used instead of ager media because embryos of peas plants improved their growth parameters