[Method verification of PTH assay according to the OLAS requirements at the laboratory of Biochemistry and Toxicology of HMIMV]

Method verification[angle qu] of analysis is a requirement of the regulatory quality standard [the Moroccan Guide of Good Execution of the Analyzes] and normative standard [the ISO 15189 standard].Mastery of this approach must be a concern for every practicing biologist. In this work, we present an example of a method verification of the determination of iPTH1-84, on ARCHITECT ci 8200® according to the recommendations of the Luxembourg Office of Accreditation and Surveillance documents [OLAS]. All the results obtained meet the pre-established analytical requirements

[Hemoglobinosis O-Arab: about three cases of incidental discovery during HbA1c assay at the biochemistry-toxicology laboratory of HMIMV in Rabat]

O-Arab hemoglobin, a rare variant of Hb related to a mutation in the HbA beta chain, is asymptomatic hemoglobinopathy in the heterozygous state and is well tolerated in the homozygous state. However, double heterozygosity or heterozygous composite can be in a severe clinical form hence the importance of diagnosis, both in genetic counseling and early management of patients. We report three cases of O-Arab heterozygous haemoglobinopathy discovered incidentally in the HbA1c assay by HPLC on ADAMS [Arkray®]

[Critical analysis of the prescription of the assay of troponine Ic]

The roponine Ic is a good tool diagnosis and forecast in the assumption of responsibility of cardiac ischemic pathologies. Its requirement in the mergency department often exceeds the framework of the international recommendations. Our objective of this work is to evaluate the practices concerning the regulation of the proportioning of TnIc. This is a study that was conducted over a period of one month from 01/04/ 2009 to 30/04/2009. Sixty-six patient who benificie of a regulation of the proportioning of the TnIc. On the whole, 130 proportionings were carried out, with the method of the first proportioning to the admission, then a second 6 to 9 hours after, in the event of negativity of the first. The reasons for admission having led to the regulation of proportioning are: thoracic pain [63%], dyspnea [18,4%], disorders of conscience [7.8%], epigastric pains [6.8%], dysarythmy [4%]. The number of pathological results of TnIc according to the symptoms of calls is of 20 cases for the thoracic pains, 3 for dyspnea, 4 for the disorders of conscience. The number of pathologicalresults of TnIc according to diagnosed pathology is of 17 per 27 cases of SCA,6 per 15 case of cardiac failure, 2 per 7 cases of ischemic heart disease, 1 per 18 cases of atypical thoracic pains and 1 per 9 cases of acute edema of the lungs. This study confirms that the proportioning of TnIc is required ill-advisedly in a large number of cases considering the percentage of pathological results [20,8%]. This proportioning as that of any biological marker is only one help with the diagnosis. These results suggests that a better control of the use of TnIc, is essential on the part of the prescribers