ABSTRACT
Catalase is the one of the most important antioxidant enzymes that is found abundantly in liver and kidney
The alteration in activity and function this latter enzyme are widely investigated in various types of cancer to understand the cancer mechanism and its treatment
The changes in the catalase activity levels in a variety of cancer cells is as a specific property of tumor tissues due to the reducting catalase activity at mRNA level
In this review, various reports that examined the alterations in catalase activity and resistance to chemotherapy and its complications in the literature are summarized and discussed
Due to the important role of hydrogen peroxide in various stages of cancer process, catalase alters this process by detoxification of hydrogen peroxide
Chemotherapy increase free radicals to destroy the tumor cells, then, catalase activity reduced their impact on cancer cells
On the other hand, it might be concluded that production of drug resistance in chemotherapy is resulted due to increasing catalase activity. Therefor it seems catalase has contradictory influence on the treatment and development of cancer
ABSTRACT
Recent studies indicate the potential role of bee venom [BV] in cancer therapy. Moreover, many evidences suggest that the regulation of apoptosis plays an important role in tumorigenesis. Considering the apoptosis-inducing and anti-tumor effect of BV, this study aimed to determine the type of the cell death induced by BV on MOLT-4 cancer cell line. In this experimental study, MOLT-4 cells were first cultured in RPMI-1640 medium in plate, then the cells were treated with different concentrations [1, 3, 6 and 8 microg/ml] of BV for 24 and 48 h. Morphology of cells, cell viability and type of the cell death were induced by BV were evaluated using inverted microscopy, the MTT assay and flow cytometry, respectively. Cell survival findings showed the BV CC[50] values of 6.3 and 0.6 microg/ ml for the cell line in 24 and 48 h, respectively. Moreover, Morphological and Annexin-V antibody analyses indicated that the BV-induced cell death might be an apoptosis. As BV can induce the apoptosis in MOLT-4 cancer cells. Thus, it would bring hope for designing novel drugs for cancer-therapy in future