ABSTRACT
Good management of the pre-analytical phase can avoid a majority of errors in medical laboratory. In this study, we made an inventory of pre-analytical non-compliances [NC] in the parasitology-mycology laboratory of Ibn Rochd University Hospital in Casablanca, in order to identify the causes and to propose corrective actions. It's a retrospective study of the pre-analytical NCs recorded in our laboratory between January and August 2016. There were a total of 546 NCs. 62% related to analysis request, 16% to inadequate sample, 15% to delivery conditions and 3% to sample identification. Each NC was systematically registered on the "KALILAB" quality software. After data analysis, corrective actions were immediately proposed. Otherwise, we organized "quality" meetings, in order to propose preventive actions. Thus, we noticed a decline in the rate of analysis request NCsfrom 10% to 5%, and in those related to inadequate sample from 14% to 7%. Other NCs were relatively stable. Pre-analytical phase is complex because of operator's multiplicity. Biologist must ensure active involvement of laboratory staff in the management process, as well as proposing corrective actions, on a larger scale, in collaboration with various partners
ABSTRACT
Flow cytometry is a cell analysis technique, after labelling with fluorochromes. It has become a useful tool for analyzing the phenotype of hematopoietic and immune system cells. Its usefulness is increasingly evidenced in the diagnosis and monitoring of haematological malignancies. It is a highly advanced technology, but still remains poorly standardized. It requires a well trained and experienced staff enough, because it may be subject to multiple sources of error which may appear at pre-analytical or analytical steps, or due to graphical acquisition problems. The interpretation of flow cytometry results is difficult and should always be attached to the clinical and biological context. The objective of this article is to present some fundamental aspects of flow cytometry technique and the main practical stages of its use in haematology through a systematic search of reference books, latest reports of expert societies and recent publications on this topic