ABSTRACT
Now a days, fever and seizure are the most reasons for admitting children in hospitals. Due to influence of genetic factors, some children undergo to the fever less than others. In addition, recent studeis have shown a positive correlation between family history for febrile convulsion [type and age of onset in child] and predisposition to this disease. Therefore, this study was performed to study the assosiation between IL1RA gene polymorphism and predisposition to the disease. In this case-control study, 100 patients affected by febrile convulsion who were referred to pediateric and emergency department of Hajar hospital were selected as case group and the control group was consisted of 130 healthy children. Peripheral blood sample [1.5 ml] was collected from the patients and DNA was extracted by standard phenol-chloroform method. Classic PCR was performed using one set primers designed for Inter Luekine 1 receptor antagonist and in the next step PCR products were analysed by PAGE [Poly Acryle Amid Gele Electrophoresis] and finally results were analysed by comparision of segments size. The average age of the patient group was 3.4 +/- 1.4 years and the average age of the control group was 3.4 +/- 1.2 years old. A positive history for febrile convulsion was detected for 44 cases of the patient group. The genotypic frequencies of the IL1RA gene allele1 and 2 in the patient group were 56% and 10%, respectively and for the control group were 55.4% and 6.9% respectively. Considering P=0.93 for allele 1 and P=0.401 for allele 2, no significance difference was found between two groups. Based on the Chi square test, there was no correlation between IL1RA polymorphism and predisposition to disease
ABSTRACT
Evaluation of extracellular matrics [ECMs] effect on differentiation of embryonic stem cells [ESCs] to pancreatic beta-cell. Mouse ESC line, Royan B1, was subjected to differentiation into beta-like cells in a three-step method: generation of embryoid bodies [EBs], spontaneous differentiation and induction by Nicotinamide onto different matrices including poly L-ornithine/laminin, gelatin, and two different dilution of matrigel [1:30, 1:100] and control group [no ECM]. At the final step, differentiated cells were analyzed for expression of some pancreas-specific genes using "semi-quantitative RT-PCR ", for detection of insulin and C-peptide presence in cells using "immunocytochemistry" and for the evaluation of the amount of secretd insulin in response to glucose Using "insulin secretion assay". The semi-quantitative RT-PCR analysis of differentiated cells on 1:30 matrigel coated-plates showed consistent higher expression of beta-cell specific markers including Insulin I, Insulin II, Slc2a2 in comparison to the other ECMs. The results of immunostainig for C-peptide showed no significant differences between the experimental groups and finally insulin secretion assay revealed that differentiated cells on 1:30 matrigel coated-plates secreted more insulin in response to glucose in comparison to the other ECMs. Our results suggest that type of ECM may influence ESC differentiation into insulin-secreting cells and 1:30 matrigel was more effective. However, the success rate of differentiation needs further investigations using other appropriate ECMs
Subject(s)
Animals, Laboratory , Extracellular Matrix , Cell Differentiation , Insulin , MiceABSTRACT
High density lipoprotein cholesterol [HDL-C] is a known inverse predictor of coronary heart disease [CHD]. Cholesteryl ester transfer protein [CETP] and hepatic lipase [HL] are key proteins in HDL-C metabolism so that decreased CETP or HL activity is associated with high HDL-C. -629C/A polymorphism in promoter of CETP gene and-514C/T in promoter of HL gene were previously reported to reduce related protein level in plasma. In this study association of these polymorphisms with CHD related to HDL-C level were investigated. In this analytical-descriptive study 321 subjects underwent coronary angiography and divided in two groups base on angiogram [non CAD = 135 and CAD = 186]. Serum lipids profile was measured by standard procedure and genotype was detected using PCR-RFLP method. Overall the CETP genotype frequencies were in CAD patients: 58.8% [n=110], 28.9% [n=54] and 12.3% [n=23] and in non CAD patients: 45.2% [n=61], 41.5% [n=56] and 13.3% [n=18] for AA, CA and CC respectively. HL genotype frequencies were in CAD patients: 61.6% [n=114], 33.5% [n=62] and 4.9% [n=9] and in non CAD patients: 65.9% [n=89], 27.4% [n=37] and 6.7% [n=9] for CC, CT and TT respectively. In control group HDL-C concentration was higher for AA than CC genotype in -629C/A, and also for TT than CC genotype in -514C/T. Allele A in all subjects and T allele in woman were higher in CAD than non CAD group. A high increase in HDL-C level [10. mg/dl] was observed in individuals with CETP-AA/LIPC-TT and CETP-CA/LIPC-TT relative to CETP-CC/LIPC-CC across all subjects [P< 0.001] but there was no difference in CAD prevalence. Allele A from -629C/A, and T from -514C/T even with the increasing of HDL-C concentration had higher frequency in CAD than non CAD group. Therefore, it seems that HDL-C didn't protect coronary artery when CETP or HL activity was reduced by these polymorphisms