Reliability of center of pressure measures during dynamic balance performance

Few studies have assessed the reliability of postural balance measures during dynamic balance performance that introduce additional challenging to postural control system. In addition sometimes in the static conditions some deficiencies of the postural control system may not be revealed obviously therefore the aim of this study was to assess the reliability of postural control parameters during functional performance on force plate in healthy subjects. Ten healthy male subjects [mean age: 25.4 years, weight: 68.2 kg height: 176.9cm] participated in this study. None of the subjects were involved in sport activities. Every subject performed three ISseconds trials of eyes open single leg stance on a force plate during dynamic balance task. Participants grasped object with hand at their waist level and release it at above shoulder level. The reproducibility of the center of pressure [COP] deviations [average speed and length of path] was assessed. All participants were tested on 2 sessions with an inter-measurement interval of 7 days. COP data was collected for each trial. The intraclass correlation coefficient [ICC] was used as parameter of intra-session and inter-session [Test-Retest] reliability. The ICCs for intra-session reliability of average speed and length of COP path were 0.89 and 0.91 respectively. The ICCs for inter-session reliability were 0.95 and 0.96 respectively. The study showed high and very high reliability for center of pressure measures during dynamic balance task. Therefore this dynamic performance can be used as a balance pattern in postural control assessment.. These can be used as reliable parameters in dynamic postural control assessment due to high reliability of average speed and length of COP path

Comparison of salivary anti helicobacter pylori IgG serum IgG with and bacteriological tests in detecting helicobacter pylori infections

This study was conducted to compare the efficacy of enzyme-linked immunosorbent assay [ELISA] for detecting anti-Helicobacter pylori [H. pylori] specific IgG antibodies in specimens of oral fluid and serum with bacteriological tests. Antral biopsy specimens, as well as serum and oral fluid samples were collected from 97 patients who underwent upper gastrointestinal endoscopy. The presence or absence of current H. pylori infection was determined by culture, histology and urease detection. Anti-H. pylori specific IgG was detected in serum and oral fluid, using an established lab-made, and a commercial ELISA kit. The obtained data were compared with results of bacteriological tests. In all, 62 [64%] of 97 patients were positive for H. pylori by one or more of the gold standard tests [culture, histology and urease detection]. Lab-made enzyme-linked immunoassay of oral fluid had a sensitivity and specificity of 92% and 83% respectively. A sensitivity and specificity of 87% and 83%, respectively, was obtained with the commercial kit. Lab-made enzyme-linked immunoassay of serum samples had a sensitivity and specificity of 90% and 88%, respectively. A sensitivity of 86% and specificity of 86% was obtained with the commercial kit. Detection of anti-H. pylori specific IgG in oral fluid by ELISA is comparable in sensitivity and specificity with serum based methods. Oral fluid based ELISA could provide a reliable, non-invasive method for the diagnosis of H. pylori infection. Saliva testing may have a role in epidemiological studies

Immunoblot assay in determination of serum antibody profile of helicobacter pylori infection

A high prevalence of antibody to cag A has been observed in H. pylori seropositive patients [HP+] living in developed countries. This is felt to be correlated with the development of serious sequelae of this infection. We examined the sero-prevalence of antibody to cag A and other specific H. pylori antigens in symptomatic and asymptomatic individuals living in Kashan. Sera from 37 patients whose HP status was determined by culture, histology, RUT, and ELISA were enrolled in this study. The Helico - blot 2.1 western blot system was used for the detection of antibodies to specific antigens of H.pylori in sera obtained from both positive and negative HP. Out of 85 patients, 47 [55.3%] were HP+ and 38 [44.7%] HP-. Among the HP+ group, 37 were tested with immunoblot system. Patients and their respective clinical disease were composed of duedenitis 19, non-ulcer dyspepsia 4, dyspepsia 4, gastritis 24, cancer and metaplasia 11 and 2 normal biopsies. In that order the corresponding antibodies to cag A was 73%,75%,75%,83%,81.8% and 50%. The results of this study suggest that cag A antibody is more frequent in the acute status; however, this factor is diagnostically insufficient in some disease processes. In addition, cag A+ phenotype can not be used as a single marker for high risk patients