ABSTRACT
Fusarium solani is the common etiological agent of fungemia and disseminated fusariosis, which is associated with high incidence of mortality in immune-compromised host. Due to high level of resistance of antifungals in Fusarium solani, rapid and specific identification of organism is essential. This study was done to evaluate the PCR method for rapid and specific diagnosis of Fusarium solani in serum samples of HIV positive patients. In this descriptive study, the PCR test based on mitochondrial cytochrome b gene as the target gene with 330 bp product was optimized. PCR was applied on 45 serum samples of HIV positive patients after evaluation of sensitivity and specificity of the test. In the optimized PCR test, the 330 bp product was amplified. The sensitivity of the test was a copy of Fusarium solani genome, and its specificity was 100%. Among 45 serum samples, 9 cases [20%] were positive for Fusarium solani. The PCR method has functional capabilities for direct, rapid and specific clinical diagnosis of Fusarium solani in HIV positive patients
ABSTRACT
One of the important indexes of efficacy in urgent department is the handling time of urgent patients. According to the American heart society studies each minute delay in CPR for urgent patient decrease 7-l0°% of survival. Others study shows 30-40% of death in casualty [victims] is in primary minutes of accidence. For improve the quality of services in urgent department shortening the transfer time and presenting good care are the subjects that should be especial attention to them. Today attention to quality of services is the goals of pioneer organizations. And in health sector because of patient life, it is very important subject that should be attention to it and improve this service continuously. This is a descriptive study that performed with documents and outcome of previous studies. According to these materials running the FOCUS-PDCA program in urgent departments has a positive effects and it decreased the patient transfer time and presenting good care. These entail the stakeholder satisfaction and quality improvement