study on risk factors of wsd outbreak in choubdeh - abadadan shrimp farming site in 2010

White spot, as one of the infectious viral diseases, has made severe losses in shrimp ponds all over the world. Despite extensive efforts made to deal with and control the disease, white spot continues to be a major health problem in shrimp farms across Iran. In this work, the significance of the risk factors of white spot disease epidemic occurred in shrimp ponds of Choubdeh farming site in Khuzestan province of Iran is determined. A cross sectional study was conducted from June 1, 2010, to September 22, 2010 in 223 shrimp ponds of the site. Data was collected on 17 variables, thought to be associated with the occurrence and epidemic of white spot, with the aid of the shrimp ponds owners and fisheries and veterinary organizations. The occurrence of white spot disease in the farming site was determined by clinical symptoms and the results of conventional PCR tests, the effectiveness of the risk factors was established by odds ratio [OR]. It is found that poor management of birds fighting [OR=3.72], less educated farm foreman [OR=3.29] and poor filtration of the intake water [OR= 3.43] are significantly affected the occurrence of the disease while little changes in the salinity of shrimp ponds [OR =0.1 6] decreases the odds of the disease. These findings help better develop shrimp farming across Iran, especially in Khuzestan province

[Purification and detection of protein band of sheep sarcocystis]

The genus of sarcocystis, zonotic parasites, have two hosts in their life cycle. They have also special importance in industrial veterinary. The serological tests are the best methods for detection of the parasite. This research was planned for isolation of sheep sarcocystis specific protein for using in serological laboratory tests. The infected muscles of sheep carcass were collected from Tehran slaughter house and transferred to our laboratory. The sarcocystis were isolated from the infected muscles and crude antigen was prepared. The crude antigen was fragmented by serial dilution of ammonium sulfate solution and followed by size exclusion chromatography. Crude antigen was electrophoresed by SDS-PAGE and its protein bands were detected by commassi brilliant blue staining. We used different concentrations of ammonium sulfate for precipitation and after by size exclusion chromatography, a 35kDa protein band was separated and observed by SDS-PAGE. The protein band of sheep sarcocystis which can be used as antigen in serological methods was parified and detected