[ effect of semen analysis factors regarding IUI with male factor infertility]

There is evidence in literature that IUI is the first line treatment and the most cost-effective procedure for mild to moderate male factor sub-fertility, however, the relative influence of various semen characteristics with the likelihood of a successful outcome is controversial. This study is designed to show the correlation between semen parameters and the pregnancy rate in patients, with mild to moderate male factor sub-fertility and whose wives were normal and underwent hyper-stimulation, including IUI. From January 2005 to January of 2006, 95 couples with male factor infertility underwent 140 IUI cycles with husbands washed semen were included in this study .They were divided into two groups based on semen parameters, mild and moderate. Post- wash sperm parameters, type of infertility [primary and secondary], male/ female age and duration of infertility were evaluated and correlated with clinical pregnancy outcome. The clinical pregnancy rate per cycle was 26 [18.5%]; 15 [21.4%] in mild group, while 11[15.7%] in moderate group. The clinical pregnancy rate per couple was 27.3%. There were significant correlation between female age, duration of infertility, sperm concentration and progressive motility, and clinical pregnancy. Our findings suggest that post- wash sperm concentration and progressive motility, female age and duration of infertility are the most important factors for prediction of successful pregnancy

[Survey of relationship between the microbial flora of cervix at time of embryo transfer and invitro fertilization [IVF] outcomes]

Introduction: The embryo transfer to uterus is the most important process with can limit the success rate in IVF. The existence of pathogenic microorganisms in cervix may lead to infection of embryo at the time of transfer

Objective: In order to determine the relationship between microbial flora of cervix at time of embryo transfer and IVF outcome a longitudinal study was conducted on infertile women in IVF center of Mirza Koochak-Khan hospital in the years of 1379-80

Materials and Methods: Thereby 100 eligible women were entered study consecutively. They underwent IVF treatment. After transferring the embryo, 2 cm of the end of using catheters were cut and put in the tubes of physiology solution, then either cultured in chocolate agar or blood agar 5% containers and were incubated for 48-72 hours in aerobic-anaerobic conditions

Results: The culture results were positive in 53 patients. The most frequent pathogen was E.Coli. The overall clinical pregnancy rate was 10% [10 cases]. This rate was significantly more in patients with negative culture than positive cultures [14.9% versus 5.7% P=0.03] and the ongoing pregnancy rate was 10.6% and 3.8% respectively [p=0.015]. The patients with positive and negative culture were not significantly different about the mean of: age, HMG consumption, serum estradiol, FSH measures and the number of embryo transferred. The mean of infertility period was different significantly, 8.9±3.8 in negative and 7.1±4.4 year in positive culture patients [p=0.03]

Conclusion: The contamination of cervix with pathogenic microorganisms at time of embryo transfer is associated with decrease success rate of IVF treatment