ABSTRACT
Background: Eremostachys laciniata is known as "Chelle-Daghi" in Iran and its rhizomes are used as an emollient to relieve rheumatoid arthritis
Objective: A phytochemical study was performed on 15 populations of Eremostachys laciniata to qualify the phytochemical variations
Methods: Plants collected from their natural habitats and dried rhizomes of E. laciniata were milled and then Soxhlet-extracted by MeOH, and then total phenols were determined calorimetrically by the Folin-ciocalteau reagent and iridoid glycosides were measured by TLC method
Results: The results of MeOH extracts showed that the highest amount with 14.85 gr/plant was obtained in the Areshtanab population. The populations had a significant effect on total phenolic concentrations [P < 0.001] and the Areshtanab with 0.281 mg GAE/g DW had higher phenolic levels than other populations. The results of TLC of iridoids showed that 15 populations had about 11 iridoids components. Cluster analysis by using Ward's method detected that the studied populations of E. laciniata were separated into three different groups
Conclusion: In summary, higher values of the total phenols and iridoids in the Areshtanab, Malek-Kian, and Til populations were considered to indicate higher levels of phytochemical heterogeneity and significant diversity between populations, which could be used to domesticate and develop the breeding programs
ABSTRACT
Because the excessive use of chemical fertilizers had harmful effects, bio-fertilizers using are suitable alternative for supply of safe food, healthy environment and human health. To evaluate the effect of bio-fertilizers on the quantity and quality yield of calendula medicinal plant. This study conducted in the research farm of Islamic Azad University [Shahr-e-Ray Branch] based on a randomized complete blocks design with three replications. Treatments were Nitroxin, Super nitro plus, Mycorrhizae, animal manure, chemical fertilizer [NPK] and control [no fertilizer]. The bio-fertilizers using had a significant effect on the most of studied quantity and quality parameters. Effect of bio-fertilizers was significant [p
ABSTRACT
Nitrogen is one of the very effective elements in increasing the phytochemical and agronomical yield of medicinal plants. Therefore it is necessary to examine the effect of nitrogen on essential oil and dry matter production of lavender. This research was carried out to evaluate changes of essential oil content and dry matter of lavender under different nitrogen treatments for reduces of nitrogen fertilizer uses. The study was conducted at the research field of Islamic Azad University [IAU], Shahr-e-Rey branch, on basis of Randomized Complete Block Design [RCBD] with 4 replication and 7 treatments. The treatments were spraying of 0, 1.5, 3, 4.5, 6 and 7.5 percent N and 180 kg ha[-1] nitrogen as urea. Different traits including plant height, stem diameter, branch number, leaves and stems dry weight, foliage dry and fresh weight, essential oil content and composition were recorded. Effect of treatments on all of the plant traits [p<0.01], essential oil content and linalool [p<0.05] were statistically significant differences. The highest amount of plant height and number of stems was observed in spraying of 4.5 percent nitrogen per hectare. Also, the maximum stem diameter, shoot fresh weight, stem, leaves and shoot dry weight, and essential oil content, linalool and 1,8-cineole was obtained in soil treated with 180 kg of nitrogen per hectare. The foliar application of 4.5% of N ha[-1] and 180 kg N ha[-1] treatments hadn't significantly effect on shoot dry weight and essential oil content. The results showed that nitrogen spraying at appropriate concentration could be improved growth and essential oil content, and also, reduced nitrogen using, contamination, and cost production