ABSTRACT
Background: Medicinal plants with biologically active components such as antioxidant and antidiabetic are useful sources of novel therapeutics. In Iran, traditionally believed that the aqueous extract of berberry fruit [Berberis integerima F.] improved health, especially in people with diabetes
Objective: In this study, in vitro phytochemical and antioxidant activities of B. integerrima and B. vulgaris fruit aqueous extracts [BIFA and BVFA] were compared. Effects of more active species on fasting blood sugar [FBS], serum lipid, and malondialdehyde [MDA] were evaluated in alloxan-induced diabetic rats
Methods: The phytochemical potential and antioxidant activity of these extracts were quantified in terms of total phenolic content and total reducing power, respectively. BIFA [500 mg/kg] and glibenclamide [2 mg/kg] were administered orally to alloxan-induced diabetic rats and FBS, body weight, lipid profile, and serum MDA were monitored at 0, 7, 14, and 21 days after induction of diabetes
Results: BIFA showed the highest phenolic content [6.816 +/- 0.132 mg/g of dry extract] and antioxidant activity [0.134 +/- 0.022 EC[50] mg/mL]. LD[50] of BIFA was found to be >2500 mg/kg. FBS, lipid profile, and serum MDA have been significantly reduced in BIFA-treated rats after 21 days versus diabetic control and glibenclamide-treated rats. Extract has significantly decreased FBS levels of rats from 138.1 +/- 1.68 to 82.00 +/- 4.02 mg/dL. Furthermore, body weight has significantly improved in treated groups
Conclusion: The results indicate B. integerrima with high phytochemical and antioxidant activities, has the potential of suppressed hyperglycemia, hyperlipidemia and lipid peroxidation
ABSTRACT
The purpose of this study was to evaluate the effects of Scrophularia striata water extract on the quality and shelf life of the rainbow trout fillet during superchilled storage. Fish samples were treated with 1% and 3% S. striata water extract and then stored at -2[degree]C for 20 days. The samples were analyzed periodically for chemical, microbial and sensory characteristics. Results indicated that incorporation of S. striata water extract on rainbow fillets caused the delay of lipid peroxidation and hydrolytic spoilage in 3% treated sample in comparison with the control sample at the last day of the experiment [P<0.05]. Moreover, fish fillets containing 3% S. striata water extract showed lower bacterial count than the control and 1% water extract supplemented samples [P<0.05] during the experiment. According to sensory analysis results, 3% treated samples were acceptable even at the end of the 20-day storage. It was concluded that the effect of S. striata extract on fish samples was to retain their good quality characteristics and extend the shelf life during superchilled storage
ABSTRACT
Various studies have been conducted to show the effects of essences and enzymes on foodborne pathogens in culture media. The aim of this study was to determine the effect of Zataria multiflora Boiss. essential oil and lysozyme alone and in combination on preventing growth of Vibrio parahaemolyticus in culture media. In this study, the MIC of Zataria multiflora Boiss. essential oil and lysozyme, together and alone, on Vibrio parahaemolyticus, was determined by using macro and microdilution methods. The MIC of Zataria multiflora essential oil determined 0.01% and 0.02% by using macro and microdilution methods, respectively and lysozyme at 1000 mg/mL concentration could not inhibit the growth of V. parahaemolyticus. According to the results of current study, Zataria multiflora essential oil showed better results in comparison with lysozyme, moreover Applying lysozyme and essential oil together did not decrease MIC, but extended the latent phase of V. parahaemolyticus, which considered an important factor in microbiology
ABSTRACT
To improve the oxidative stability of meat products, the use of the dietary form of natural additives, especially those with plant origin is increasing. Aloe vera plant, the in vitro antioxidant effect of which has been previously discussed, is a potential candidate for this purpose. This study was designed to evaluate the effects of feed supplementation with Aloe vera gel extract on lipid peroxidation of broiler breast fillets during frozen storage. Fifty-four 1-day old broilers were allocated into three groups [basal diet as control, basal diet supplemented with 100 and 300 mg/kg methanol extract of Aloe vera gel] and fed for 6 weeks. In the term, chicks were slaughtered and their breast fillets were stored at -20[degree sign]C for 9 months. Lipid peroxidation was assessed after 1, 3, 6 and 9 months of frozen storage using chemical [PV and TBARS] and sensory evaluations. Results indicated that incorporation of 300 mg/kg Aloe vera gel methanol extract in broiler diets caused the delay of lipid peroxidation in raw breast meat [with 9.6 meq/kg, 92.67 micro g/kg and 6.3 in PV, TBARS and Sensory evaluations, respectively] in comparison with control sample [with 15.2 meq/kg, 139.33 micro g/kg and 3 in mentioned evaluations] at the last day of the experiment [p<0.05]. This study showed that methanol extract of Aloe vera gel can be considered as a dietary supplementation substance in chicken diet and can delay the oxidative spoilage of chicken breast fillets during frozen spoilage
Subject(s)
Animals , Lipid Peroxidation , Plant Extracts , Dietary Supplements , Frozen Foods , Meat , Antioxidants , ChickensABSTRACT
Poultry products have been recognized as major sources of human gastrointestinal disease caused by Salmonella spp. and several outbreaks have been reported where eggs were the source of human infection. This study was carried out to determine the prevalence of Salmonella spp. in the quail egg interior contents from retail stores of Semnan, Iran and to characterize the isolated Salmonella serovars via serotyping and Multiplex PCR techniques. 140 packages of quail eggs [each package containing 12 eggs] were collected from different batches during summer 2010 and tested for the presence of Salmonella through conventional culture and serotyping methods. From these samples, S. enteritidis was detected in the egg contents of one package [0.71%] out of 140 packages. This isolate was confirmed by Multiplex PCR generated amplification products for a random sequence that is specific for the genus salmonella and spv and sefA genes. According to our results, S. enteritidis is the most prevalent serotype of quail egg content contaminant in the Semnan area of Iran and the multiplex PCR method could be used as a reliable method of identifying Salmonella serovars
ABSTRACT
Due to the range of its constituents, colostrum has been considered as a supplement for various diverse purposes. This study was conducted to examine the effect of supplementary bovine colostrum on serum malondialdehyde [MDA], antioxidant activity [AOA] and glucose in a diabetic rodent model. Sixty male Wistar rats were divided into 10 groups of six rats each for 40 days as follows: non-diabetic; diabetic; diabetic with 10%, 20% or 30% colostrum intake; non-diabetic with 10%, 20% or 30% colostrum intake; diabetic treated with insulin; and diabetic treated with glibenclamide. Although serum MDA levels showed a significant decrease in response to insulin [2.56 +/- 0.31 umol/L] and 10%, 20% or 30% colostrum intake [0.46 +/- 0.04, 0.29 +/- 0.06, 0.37 +/- 0.09 jumol/L, respectively], the decrease was greater in the diabetic rats [3.92 +/- 0.29 umol/L] [p < 0.01]. Significant changes were seen in the AOA of both insulin [0.78 +/- 0.11 mmol/L] and glibenclamide [0.7 +/- 0.08 mmol/L] treated rats compared to the diabetic rats [0.69 +/- 0.1 mmol/L]; however, AOA showed a significant increase in response to 10% [1.78 +/- 0.11 mmol/L], 20% [1.57 +/- 0.02 mmol/L] and 30% [1.75 +/- 0.02 mmol/L] colostrum [p < 0.001]. All treated groups showed a significant decrease in serum glucose levels compared to the diabetic group [391 +/- 39.79 mg/dL] [p < 0.01]. It seems that colostrum might be a beneficial dietary supplement for reducing serum MDA and glucose levels while increasing serum AOA in type 1 diabetes mellitus