[Histomorphology of the olfactory mucosa and spinal tissue sparing following transplantation in the partial spinal cord injury in rats]

Nowadays, cellular and tissues transplant has become the focus of attention for spinal cord injury. It has been shown olfactory nerve cells or olfactory mucosa whi have more efficient on nervous tissue repair and they have been more studied in experimental study. Furthermore, they were used in a few clinical centers for spinal defect. But mucosa tissue and spinal tissue have different structure and there is doubt about the integration of mucosa tissue in nervous tissue. Thus, in this research the morphology and the effect of the fetal olfactory mucosa [FOM] on spinal tissue sparing were studied after transplanted into the spinal cord hemisection in rats. This experimental study was conducted at Iran university of Medical Sciences in 2008. Of thirty eight female Sprague-Dawley [200-250g] rats twenty- eight were spinally hemisected at the L1 spinal level and were randomized into two groups of 14 animals. Treatment group received FOM graft and the control received fetal respiratory mucosa graft [FRM]. The other animals received surgical procedure without spinal cord injury as a sham group. The morphology of the transplant region and spinal tissue sparing was examined histological eight weeks after transplantation. The collected data was analyzed by the SPSS software using ANOVA and the morphology of the transplant region were studied by light microscope. Histological study showed that the both mucosa tissues could not integrate with the parenchyma of the spinal tissue. Although the FOM were fused more then the FRM with the host tissue but clear boundary was seen at the graft-host interface. The mean spinal tissue sparing of the treatment group increased a little compare to the control but a significant difference was not apparent whereas, the spinal tissue sparing in treatment and control groups compare to the sham group decreased significantly [P <0.05]. Transplantation of the mucosa tissue directly, into the spinal cord injury was created different cytoarchitecture with spinal tissue and FOM partially preserving tissue sparing

[Studies on the effects of turpentine powder on the serum concentration of lipids and lipoproteins of female rabbits]

To Study the effects of turpentine powder on the concentration of lipids and lipoproteins of rabbit [as an animal model for human studies].Experimental Study.30 healthy female rabbits.30 healthy female rabbits were chosen and thenrandomly allocated into 6 groups of 5 rabbits each. Differentpercent ages of turpentine powder [10% and 100%] and oilcompounds [vegetable oil, cholesterol and animal oil] weregiven to each group, separately. Blood serum lipid andlipoprotein levels were analyzed using routine laboratorymethods.Analysis of variance and Duncan'smultiple range test.Treatment with 10% and 100% turpentine powderhad significant decreasing effect on Serum triglyceride, cholesterol, total lipid, VLDL- cholesterol and LDL-cholesterol [P<0.05] Moreover, it significantly increasedthe Serum HDL- cholesterol [P<0.05]. Treatment with 10%vagetable oil had a significant increasing effect on the serumtriglyceride and VLDL-cholesterol [P<0.05]. Followingtreatment with 10% animal oil, the concentration of HDL-cholesterol showed a significant [P<0.05] decreas while theconcentration of cholesterol and LDL-cholesterol showed asignificant increase [P<0.05].After treatment with 1%cholesterol, the concentration of serum HDL- cholesterol, LDL-cholesterol, VLDL-cholesterol, triglyceride and totallipid significantly increased [P<0.05].Treatmentwithturpentinepowderhassignificant effects on the levels of serum lipids andlipoproteins