ABSTRACT
Background and Aim: Salmonella spp. is important in medicine. Most related infections are self-limited. Antibiotics are used for high risk patients such as children, old individuals and immune-compromised patients. Overuse of antibiotics has led to increased emergence of multi-drug resistant bacteria which are life-threatening. Phage therapy is an alternative method to reduce antibiotic use
Materials and Methods: Waste water samples were collected from sewage system of Rasht City twice a month. Prepared suspensions of Salmonella reference collection A [SARA] number 1, 6 [S. typhimurium] and 46, 48, 51 [S. paratyphi B] and double LB broth were mixed with filtrated waste water. After centrifugation and filtration, serial dilutions were prepared and phages were isolated. Morphologic characteristics were determined using TEM [Transmission electron microscopy]. Finally, to assess the spectrum effect of the bacteriophages, we determined host range against 19 SARA strains
Results: Clear plaque formation on double layer LB agar indicated lysis of the test strains by isolated phages. The results of host range showed that some of the phages were able to lyse a number of other bacteria of SARA collection. Imaging with TEM indicated that the isolated phages against SARA collection belonged to Siphoviridae and Podoviridae families
Conclusion: This is the first report of phage isolation against Salmonella reference collection in Iran. SARA no. 51 [S. paratyphi B] was lysed by S. typhimurium phage. It means that the isolated phage may lyse S. typhi, which is an important human pathogen
ABSTRACT
Appropriate diagnosis in oral medicine requires expertise, experience and the use of several other related fields. One of the important fields in this domain is oral pathology. The purpose of this study was to investigate the consistency of clinical diagnosis and pathological reports of oral lesions in patients referring to oral pathological department of Kerman dentistry school during 1995- 2004. The present research is a descriptive- analytic and retrospective study in which all of the samples referred to the pathology department in Kerman dental school during 1995 to 2004 were considered. The corresponding data were analyzed by Chi-Squire and T tests using SPSS 13.5 program for statistical analysis. The histopathologic finding and clinical diagnosis were in the same line in 432 cases [64.87%] The highest percentage of proper diagnosis was found in the eight decade with the location in gingival lesions .The lowest percentage of agreement was found in the third decade and in the lesions of mouth floor. Based on the findings of the present research, it seems that the clinical diagnosis is not consistent with pathological finding in about one third of cases. Therefore, the students should be provided with sufficient information about the importance of careful recording of the patient's characteristics and documentation of oral lesions in the charts
Subject(s)
Humans , Jaw Diseases/diagnosis , Mouth Diseases/pathology , Jaw Diseases/pathology , Retrospective StudiesABSTRACT
Odontogenic keratocyst [OKC] and dentigerous cyst are two most common developmental odontogenic cysts involving jaws. OKC has a high growth potential in contrast to its minor clinical manifestation. It has also a high degree of recurrence rate. The purpose of this study was to diagnose the OKC and dentigerous cysts and also differentiate them based on their immunohistochemical criteria. To provide the materials for this research, we preliminarely reviewed the records of the patients referred to pathology lab of shahid Beheshti Dental College between March 1999 and March 2003 and 60 paraffin-embedded biopsies [20 OKC, 20 dentigerous cysts and 20 dental follicles] were selected. Then immunhistochemical staining was applied to identify cytokeratin-10 [CK-10] and cytokeratin-19 [CK-19] and their conditions, as well. The expression of CK-10, CK-19 are seen in OKC and dentigerous cyst. CK-10 was found more in OKC, along with CK-19 in dentigerous cyst. The expression of these cytokeratins was more in upper half of the epithelial lining of these cysts. One of the dental follicles expressed CK-19, but not CK-10. CK-10 is more specific for OKC as well as CK-19 for dentigerous cyst. These cytokeratins are usually seen in the upper layers of the cyst lining. If an expression of cytokeratin-19 is found in a dental follicle, it may correspond the cystic transformation of the latter one