Therapeutic effect of bone marrow stromal cells and coenzyme Q10 on rat models of parkinson's disease

The assessment of the ability of combined treatment of bone marrow stromal cells graft [BMSCs] and oral administration of Coenzyme [CoQIO] in rat model of Parkinson disease as a good substitute for common current Parkinson treatments, and the comparison of this combined treatment method with alone application of these treatments. In this experimental study of male Wistar rats were used. They were divided into six groups: control, sham, lesion, treatment groups with oral administration of CoQIO, treatment with graft BMSC and combined treatment with graft BMSC and oral administration of CoQIO. Oral administration of CoQIO with 200 mg/kg/daily dose started a week before the model creation procedure and continued throughout the whole treatment period. The laboratory model of Parkinson disease in rats was performed by injecting 2.5 microlitre saline solution 0.9% containing 8 micrograms 6-hydroxy dopamine [6-OHDA] and 0.2% ascorbic acid in substantia nigra pars compacta. Also in sham group the same volume solution saline-ascorbic was injected. BMS Cells were labeled by 5-Bromo-2'-deoxyuridine [Brdu] before transplantation. Behavioral assessment before creating the model, two weeks after creating the model and eight weeks after cell transplantation was performed. At the end of second month of treatment, Immunohistochemistry and histology Studies were performed. Behavioral assessment of two groups of alone treatments indicated the equal recovery in comparison with lesion group [p<0.01] while combined treatment of BMSC and Co Q10 showed a considerable recovery compared with lesion group [p<0.001]. In addition according to histological studies, no sign of gliosis and graft rejection was seen. Immunohistochemistry studies of Brdu indicate that the cells are alive after two month of application in host tissue. Cell count assessment showed that the number of neural cells in combined treatment of BMSC and Co Q10 was significant difference with others experimental groups [p<0.001]. The combined use of two neuroprotective treatment and replacement therapy can have a more effective role in the treatment of Parkinson's disease in comparison of alone treatment protocols

[ effect of ethanol on growth and development of two-cell arrested mouse embryo]

Arresting in certain step of developing like two cell block, could be the reason of infertility in some couples. Evaluate the effect of ethanol on growth and development of mouse two-cell arrested embryo is aimed of this study. In this experimental study 4-6 weeks old female mice were coupled with male mice following superovulation. Positive vaginal plaque mice were killed 48 hour after HCG injection. Two cell embryos were collected in RPMI medium and cultured in M16 medium and divided in three groups. The 2[nd] and 3[rd] groups were exposed to 4[degree sign] [c] for 24 hour in order to delay and arrest for cleavage and developmental rate. The 2[nd] group [2[nd] control] were incubated immediately, while the 3[rd] group [experiment] were exposed to%0.1 Ethanole for 5 minutes and the 1[st] group [1[st]control] without any exposure to low temperature were incubated. The developmental rate of embryos exposed to low temperature [4[degree sign][c]] significantly decreased and induce, retardation and arrest [p=0.001]. There were not significant difference between the groups mean of cleavage rate, but the mean percent of degenerated embryos between groups have significant differences [p=0.045]. The mean percent of morulla were significantly different between groups [p=0.005]. The mean percent of blastocyst and hatched blastocyst after 120 hr evaluation have significant differences between others groups [p=0.014] [p=0.001]. Effect of%0.1 ethyl-alcohol on arrested two cell embryos can significantly increase the mean percent of morulla and development of blastocyst and hatching blastocyst stage in compare to control group, without any significant effect on cleavage rate