ABSTRACT
Diabetes mellitus is one of the most common chronic diseases around the world with increasing prevalence in Iran. Weakening of the antioxidant defense system that accompanies this illness may lead to several complications. In this study, we evaluated the parameters of oxidative stress in patients with type 2 diabetes and compared them with healthy individuals. In this cross-sectional study, 30 patients with type 2 diabetes who referred to diabetes clinic in Arak city were included based on well-defined inclusion criteria. They were compared with 30 individuals as the control group who were matched by age and sex. The total antioxidant capacity of serum were measured by Ferric Reducing Ability of Plasma technique in all participants. The ThioBarbituric Acid method was used to estimate the amount of lipid peroxidation and finally, HU method was used to estimate the protein oxidation [Thiol groups]. The data were analyzed with SPSS software. The mean +/- SD of total antioxidant capacity of serum in patient and control group were 2.743 +/- 0.08092 micro m/ml and 1.927 +/- 0.04830 micro m/ml respectively [P<0.001]. The mean +/- SD of lipid peroxidation in patient was control group were 3.2596 +/- 2.0650 microm/ml and 3.9050 +/- 3.5354 microm/ml, respectively that it was not statistically significant. The mean +/- SD of protein oxidation [thiol] in patient and control group were 0.2038 +/- 0.1307 and 0.3768 +/- 0.1487 micro m/ml respectively [P<0.001]. Our findings showed that the increased amount of total antioxidant capacity of serum in diabetic patients was meaningful, but lipid peroxidation was not significantly different between two groups that can be caused by taking natural and medical antioxidants by patients. The production of free radicals in patients has been increased and therefore the antioxidant defense system has been weakened