ABSTRACT
Objective: Carboprost is used to prevent postpartum hemorrhage in pregnant patients but is associated with severe nauseaandvomiting. This a study was done to compare ondansetron and P6 point acupuncture stimulation for prevention of carboprost induced nauseaandvomiting. Methodology: Fifty ASA class I and II full term pregnant patients scheduled for elective cesarean section were r and omly allocated into Group-O [n=25] and Group-P [n=25]. Subarachnoid block [SAB] was administered in both groups using inj.0.5% hyperbaric bupivacaine in L3-L4 interspace to achieve a level between T4 to T6. Inj.carboprost 250 mcg was given intramuscularly after the delivery of anterior shoulder in both groups. In addition Group-O inj. Ondansetron was given and in Group-P acupuncture was applied at P6 acupoint. Mean arterial pressures were maintained within 20% of baseline. Both groups were observed for nauseaandvomiting for two hours postoperatively. Results: Overall incidence of nauseaandvomiting was significantly less in Group-P than in Group-O, however, there was no significant difference in incidence of nauseaandvomiting among the two groups when considered individually. Conclusion: We conclude that stimulation of P6 point by acupuncture in comparison to ondansetron decreases the overall incidence of carboprost induced nauseaandvomiting in the immediate postoperative period. Key words: Carboprost, Cesarean section, Nauseaandvomiting, P6 acupuncture, Ondansetron
ABSTRACT
Todevelop a new high-performance thin-layer chromatography [HPTLC] method for the quantification of berberine in herbal extract and pharmaceutical dosage form. The HPTLC was performed on aluminium foil plates coated with 200 mm silica gel 60F254. Linear ascending development with toluene:ethyl acetate:formic acid:methanol 9:9:3:1 [v/v/v/v] was performed at room temperature [25 +/- 2 C] in a twin-trough glass chamber saturated with mobile-phase vapour. Compact bands [RF 0.58 +/- 0.02] were obtained for berberine. Spectrodensitometric scanning was performed in fluorescence mode at 350 nm. The method was validated for precision, recovery, robustness, specificity, and detection and quantification limits, in accordance with International Conference on Harmonization guidelines. Linear regression analysis of the calibration plots showed a good linear relationship [r2 = 0.9996 +/- 0.0001] between peak area and concentration in the range 10-100 ng/band, respectively. The limits of detection and quantification were 2.8 and 9.3 ng/band. The recovery of the method was 98.5-100.6%. The above method was a rapid and cost-effective quality-control tool for routine analysis of berberine in herbal extracts and in pharmaceutical dosage form