effect of FBS concentration on cryopreservation of isolated spermatogonial cells from neonatal mouse by MACS method

The purpose of this study was the separation of spermatogonial cells from prepubertal NMRI mouse testis by magnetic activating cell sorting [MACS] method and assessment of FBS concentration on surviving these cells after cryopreservation. Testes from 6-days-old mouse removed and digested in two enzymatic mediums, first 8-9 min at medium containing collagenase, trypsin and DNase and second 10-12 min in medium containing collagenase, trypsin, DNase, hyalloronidase and EDTA and then the spermatogonial cells were isolated by [MACS] method. In the freezing step, the cells were frozen in three medium containing DMEM/F12 medium, 10% DMSO and 50%, 60% and 70% FBS serum, named group I, II.III. The viable cells obtained after enzymatic disaggregation were 91.66% +/- 0.60% and after being isolated by MACS method were 95.25% +/- 0.33%. The purity of the isolated cells was 93.79% +/- 2.20%. The cells frozen in group I, II and III had 39.09% +/- 0.15, 85.55% +/- 6.98 and 90/29 +/- 1/38% viability, respectively. according to the obtained results, the increase of temperature at digestion step reduces the time of testicular tissue disaggregation and consequently increase viable cells. Higher viable cells and purity can be attained by using of a6 integrin and magnetic beads. The results show that spermatogonial cells in freezing media containing 60% - 70% FBS have the highest viability after thawing

comparison of delayed and early diagnosis of operative results gained through internal fixation of lateral humeral condyle fractures in children

Lateral humeral condyle fracture is one of the most common fractures during childhood. Delay in operation and internal fixation often associated with hopeless treatment results. The goal of this study is comparison of complications and operation outcomes between the patients that underwent early operation with the patients that undervent delay operation. This study was prospective and case series, performed on 60 patients from may 1998 to 2004. The patients were divided into two groups. The first group included 30 patients, treated during first week after lateral humeral condyle fracture, and the second group included 30 patients treated between 7 to 28 days after fracture. Method of operation was similar for the two group patients. All of the patients were studied for 12 to 30 months after treatment. In the first group, 16.66% were diagnosed to have Milch I and 83.34% Milch II fracture, As for displacement, 10% of the patients were in stage I, 40% stage II and 50% stage Ill, in the second groups 20% of fractures were diagnosed to have Milch I and 80% Milch II. As for displacement, 13.3% of patients were in stage I, 33.3% stage II and 53.4% were in stage III. Most of the patients in the first group were in excellent conditions considering the range of movement of elbow; however, in the second group they were mostly in moderate and poor conditions. The most common cause of delay in treatment was fracture handling by bonesetter. Delay exceeding 1 week in treatment of lateral condyle fracture will be followed by numerous complications. To prevent delay in operative treatment the public culture for early and true treatment must be reformed

Missed injuries in patients with multiple traumas in Shohada hospital

Delayed diagnosis of musculoskeletal injury in multitrauma patients may potentially cause functional or cosmetic sequelae remaining for the rest of survivors life. Identification of factors which hide injuries from early diagnosis in multitrauma patients will decrease their occurrence and prevent many functional disabilities and cosmetic sequelae. We planned this study to assess the reasons for injury concealment in multitrauma patients and provide resolutions to reduce them. This cross-sectional study on 487 patients was carried out in Tabriz Shohada Hospital from February 2004 to February 2005. After collection of needed data, a questionnaire was filled for every multitrauma patient who had a missed injury. The relative frequency of multitrauma was 18.51% among all studied trauma patients [487 cases], but 7.6% of included patients [28 from 367 trauma cases] had missed injury [ies]. 32 missed injuries were found in 28 patients. Most injuries were musculoskeletal, involving the distal part of the limbs [feet, hands, wrists and ankles]. Fracture was the most common type of musculoskeletal injury [19 out of 32 missed injuries]. Causes that lead to concealment of injuries were founded to be inadequate physical and / or, radiographic examinations, commonly after severe penetrating injuries which are mostly due to motorcycle accident. Most of the missed injuries have been ultimately diagnosed upon the patient's complaints in hospital wards, within 1 to 30 days after trauma. We can prevent missing of injuries by complete and accurate physical examination and perfect radiographic evaluation according to the standard protocols. Repetitive serial examinations during admissions and after discharge can reveal missed injuries

[ study of factors and kinds of acute occupational injuries in Shohada Hospital]

Tools and instrument were developed with progress and development of societies. Whereas inattention to acquire of correct principles of job with these new tools may increased injuries in agriculturists and workers. Occupational hand injuries are one of the most common causes of time lost from work in agriculture and industry. The goal of this study was to investigate the prevalent causes bringing about acute hand injuries in accidents resulting from work in the above mentioned professions and also types and intensity of the injuries caused in the suffering workers and farmers and the time of absence from work. This is a discreptive prospective study. The population that was selected for this included 1130 case. Patients managed for acute occupational hand injuries during summer 1999- 2003 in Shohada hospital and patients assayed in view of kinds and factors of injuries. From 1130 patients, who admitted in Shohada hospital, 405 cases [35.8%] were related to agriculture injuries and 725 [64.2%] cases industrial injuries. The most common causes of agriculture injuries was lawn mower [38.3%] and the seconde cause was fall of heavy material [29.6%]. In industrial injuries the most common cause was fall of heavy material [38.3%], the seconde was press or turnery machins [31.7%]. The most victoms were in the first or seconde decade of life. Average time of hospitalization was 5 days [1-24 days], and average of follow-up time was 2 months [1 weak- 5 month]. We concluded that inadequate experience, training and protective devices incraese the possibility of occupational injuries