ABSTRACT
Background: brucellosis is one of the five common bacterial zoonosis caused by a gram negative, non-spore forming, and facultative intracellular bacterial organism belonging to the genus Brucella. Although brucellosis is considered as a health problem for both men and domestic animals in many countries, any licensed human vaccine has not been designed and produced for it yet. To overcome the problem, currently, antigenic determinants of Brucella cell wall e.g. outer membrane proteins [OMPs] and lipopolysaccharide [LPS] are considered as potential candidates to develop subunit vaccines
Materials and Methods: brucella abortus S99 used in the present study is obtained from the standard bacterial collection of Institute Pasteur of Iran. OMPs were extracted by deoxy cholate extraction technique and further purification performed by sequential centrifugation and ultracentrifugation. Protein concentration was determined using the Nano drop ND-10000 spectrophotometer. SDS-polyacrylamide gel electrophoresis [SDS- PAGE] was performed to determine the electrophoretic pattern and the molecular weight of the extracted OMP samples
Results: OMPs concentration of B.abortus S99 has been measured and reported as 6.27 mg/ml. SDS-PAGE analysis indicated one protein band in the range of 36-38 kDa which would be classified as the porins of B.abortus S99
Conclusion: extraction of B.abortus S99 OMPs with the applied method in the present study produced a satisfactory yield of OMPs. These proteins belonging to the second group of OMPs, called porins
ABSTRACT
The radioprotectory effects of vitamins A and E post and past Gamma-irraiation on mouse bone marrow cells using micronucleus assay was analyzed. Micronucleus assay is a very fast and effective method in evaluating, structural as well as numerical chromosomal abnormalities. Search for find/ng an effective way of protection against harmful effects of induced or spontaneous irradiation is actively progressing. Effects of antioxidants such as vitamin A and E in in vivo have studied extensively. In this study effect of different doses of vitamin A and E post and past Gamma-irradiation of 2 Gy wererepresenting chromosomal damages. Of all doses used [0.6mg/kg, 1.2mg/kg and 2.4mg/kg for vitamin A and 50mg/kg, l00mg/kg and 200mg/kg for vitamin E/ l.Amg/kg, body weight of vitamin A and 200mg/kg body weight of vitamin E had the best effect on reducing induced-damages. Combination of two vitamins in these doses post and past irradiation revealed that vitamin E was the most effective post irradiation whereas vitamin A was the most effective past irradiation in reducing the frequency of micronucleus.According to our knowledge this is the first time that the anticlastogenic effect of vitamin A and E post and past irradiation in in vivo has been studied at the same time using micronucleus assay