Trials with avian anticoccidials for treatment of coccidiosis in lambs

Twenty Baladi lambs aged 3-4 months were found naturally infected with coccidia. Six species of Eimeria were identified, which are E. ninakohlyakimovae, E. parva, E. intricata, E. granulosa, E. cr and allis, and E. ovina. Trials for treatment of such infestation were made using one old traditional anticoccidial drug [Amprolium] and two recent preparations, which are AS supermed [combination of Amprolium and sulphaquinoxaline], Baycox [Toltrazuril]. The criteria used for the efficacy of such drugs are oocyst discharge and body gain. It was found after the statistical analysis that, Amprolium is still the drug of choice in treatment of coccidiosis in lambs. On the other h and, the efficacy of Baycox and AS supermed is quite equal in decreasing oocyst discharge and increasing body weight of treated lambs

Direct immunofluorescence and immunoperoxidase techniques to study the development of rinderpest virus in vero cells and their utility to detect antibodies to rinderpest virus in vaccinated calves

The development of Rinderpest virus [RPV] in Vero cells was studied using direct immunofluorescence [IF] and direct immunoperoxidase [IP] techniques 6, 12, 18, 24, 36, 48, and 72 hours post inoculation of Vero cells with Kabete 0 strain of RPV. Coverslides were fixed and examined with both IF and IP techniques. RPV antigen could be detected 6 hours post inoculation by the direct IF test, while it could be firstly detected 12 hours post inoculation by the direct IP test. Syncytia were observed 48 hours post inoculation. The application of this study was carried to improve the traditional neutralization test. The detection of residual virus activity in cell cultures after neutralizing procedure was done by neutralizing immunofluorescence [NIF] test and by neutralizing peroxidase linked antibody [NPLA] test. Serum samples were collected from calves immunized with the locally prepared TCRR-vaccine and were tested with the abovementioned techniques for detection of neutralizing antibodies to RPV. Statistically there was no significant difference between the two techniques, while NPLA is easier in its application, needs less material and can be done on a large number of sera at a time. Thirteen out of 15 calves had the protective level of antibodies to Rinderpest virus after vaccination with the locally produced TCRV