[Effect of cobalt sulfate intraperitoneal injection on the quantitative values of the kinds of white blood cells and initiation of liver fibrosis in rat]

Background: cobalt 60 is used in sterilization and there are many other variable uses of cobalt sulfate in the synthesis of alloys in air-space army dependant manufactures and also regard of its domestic uses and importance of cobalt soils after bombardement we decided to investigate this metal. And because of WBC involement in many important system 'disorders we decided to investigate effect of cobalt sulfate on WBC amount

Materials and Methods: in this experimental study:47 four weeks old wistar rats with body weight 224[male] and 1493[female] were purchased from Pasteur Institue. One month was considered for their adaptation with animal laboratory with 20-26 centtigrade and 12hour dark and 12 hours light cycle.The animals were divided in every sex into 5 groups.4 Doses 1.0, 3.5, 8.0, 11.5miligramper kilogram body weight were prepared and were administered intraperitoneally for 1, 2, 3and 4 groups.The fifth group receive distilled water with the same injection rhytme [2 mililitres, three times in the week]. After 9 weeks they were anesthetized and blood from heart and bone marrow from the femur were sampled.The livers of the rats were dissected free and prepared for light microscopy. Their blood parameters were measured and the smears of bone marrow and blood were stained with Wright and Giemsa stain. Livers were stained by Hematoxicilin andEosin and Van Giesson stain. The slides were studied and the results were analysed with Minitab software and Mann Withney test

Results: eosinophil, basophil did not have any significant change in the groups.Liver pathologic figures like fibrosis and leukocyte infiltration has been seen in the fourth male group[P=0.02]

Conclusion: the precise analytical changes of WBC in this exposure needs to study more in high quantityThe results presents the possibility of WBC increase

[Reapair and Remodeling of Epithelial Tissue with Cell Culture in Vitro]

Background: epithelial is an important tissue in our body. The human epithelium forms a barrier between the external and internal environment

Materials and methods: this study was a Lab-Experimental. We were used 4 male albino rabbits in this study. At first, 6×6 cm[2] full thickness skin separated of rabbit and cultured in DMEM and FCS 10%. Then 2×1 cm[2] skin separated of other rabbit and cut into small pieces and transplanted on the first skin's derm. After 2 weeks, small pieces covered the entire feeder layer and then new skin grafted on the donor rabbits and supported for 2 weeks. Then after, histological studies were done

Results: histological studies were shown epidermal layer were normal but the number of layers were lower than normal skin. Other findings were thin cornified layer, low skin appendages, absent of sweat gland, regular of collagen fiber in dermal layer and small rete ridge

Conclusions: with this method of cell culture, skin can expand 18 times and a normal skin was obtained