[Study of glycoconjugates terminal sugars during gonadogenesis in rat by lectin histochemical method]

Gonadogenesis is a complex developmental process which is regulated by molecular interactions such as cell surface and extracellular matrix [ECM] Glycoconjugates terminal sugars. The aim of this study was to determine Glycoconjugates terminal sugars expressions and changes during Gonadogenesis in Rat using Lectin histochemical technique. To determine of glycoconjugate terminal sugers by Lectin histochemistry method, thirty Wistar Rat embryos at different stages from days 12 to 16 of gestation were fixed in formalin, embedded in paraffin and cut in to 5microm thickness sections serially. The sections were incubated with different HRP- lectins from Lotus tetragonolobus [LTA], Maclura pomifera[MPA] and Arachis hypogaea or Peanut [PNA], that are specific for terminal sugars L-Fuc [alpha2-4]GlcNAc, Gal[beta1-3]GalNAc and D-Gal[beta1-3]DGalNAc respectively. On the basis of colorimetery data that was determined by blind's method, sections were graded and SPSS statistic soft ware and kruscal Wallis tests were used for comparing different embryonic stages. Our finding showed that LTA did not react with developing gonads at gestational day 12[E12]. LTA reactivity was found in extracellular matrix [ECM] of developing gonads from E14 through E16 [P<0.05] and PGCS as well as surface epithelium at E16 [P<0.05]. Surface epithelium were reacted with MPA from E12 to E14 and increased LTA reactivity at E16 [P<0.05]. ECM was reacted with MPA at E14 and diminished at E16 [P<0.05]. LTA reactivity was found in PGCS at E16. PNA did not react with developing gonads at gestational day 12[E12] but its reaction was started with PGCS from E14 and increased to E16 [P<0.05]. In addition, PNA reactivity was started with ECM and surface epithelium from E14 and increased at E16 significantly [P<0.05]. According to our result, it is concluded that the expression and changes of glycoconjugates with terminal sugars L -Fuc [alpha2-4] GlcNAC, and D-Gal [beta1-3] DGalNAC are regulated developmentally during rat gonadogenesis

[ rare anatomical variation of sciatic nerve and its relationship with piriformis muscle]

Sciatic nerve is the thickest nerve of the body which is formed by ventral branches of L4, L5, S1, S2 and S3 of spinal nerves as a part of sacral plexus in pelvis. It leaves the pelvis via the greater sciatic foramen below piriformis muscle and descends between the greater trochanter and ischial tuberosity, along the back of the thigh and dividing into the tibial and common peroneal [fibular] nerves, at a varying level proximal to the knee. In addition, the piriformis muscle, the intrapelvic muscle, may compress the sciatic nerve and causes the piriformis syndrome. At present case, sciatic nerve was divided in the pelvis and left the pelvis via supera piriformis, inferapiriformis and interapiriformis. According to the reports about the variations of sciatic nerve regarding to its leaving and dividing point, it is important in surgical operations, muscular injection in gluteal regions and piriformis syndrome