phylogenetic study on the NP gene of detected canine distemper virus in [2008-2011] Iran

Canine Distemper [CD] is a lethal systemic disease affecting a wide variety of terrestrial carnivores. This study was performed to survey epidemiological and molecular characteristics of the canine distemper virus [CDV] strains circulating in Iran. In this study, 19 CDV-suspected unvaccinated dogs from Northeast and center of Iran were analyzed for presence of CDV nucleoprotein [NP] gene using Nested-PCR during 2008- 2011. Different biological samples of 14 dogs were positive. The phylogenetic analysis based on partial NP gene sequences indicated the presence of two major clusters that are clearly different from vaccine strains in Iran. One cluster belongs to the European group and the other one to the Arctic group. Due to a lack of phylogenetic analysis on CDV in countries bordering Iran, except Turkey, the ancestor of Iranian sequences specially Iranian Arctic sequences could not be definitely identified. This study is the first report on phylogenetic analysis of CDV from domestic dogs in Iran

Phylogenetic study based on the phosphoprotein gene of Iranian Newcastle disease viruses [NDV] isolates, 2010 -2012

Newcastle disease virus [NDV] is the causative agent of the Newcastle disease [ND], a highly contagious disease in birds that causes significant economic losses to the poultry industry worldwide. ND is endemic in Iran and outbreaks are reported regularly in commercial poultry flocks and different species of birds. The current study was carried out to characterize NDV based on phosphorprotein [P] gene from recent outbreaks in Iran, 2010-2012. The P gene fragment of NDV isolates of five chickens, 1 ostrich, and 1 Pigeon paramyxovirus-1 was obtained by RT-PCR and sequenced. Phylogenetic analysis of sequences revealed that chicken and ostrich NDV isolates were closely related and placed in the genotype VII and Pigeon Paramyxovirus-1 was located in the genotype V. This is the first report of Phosphoprotein gene sequences of NDV strains isolated in Iran. This study will help us to understand the epidemiology and molecular characteristics of Newcastle disease virus in Iran