Evaluation of the recombinant m9 protein as haemagglutinating antigen for serodiagnosis of M. gallisepticum in chickens

This study focused on a prominent haemagglutination feature of the pathogenic M. gallisepticum, which have agglutinins that are immunogemc surface protein. M9 protein was prepared using cloning and expression technology and evaluated as haemagglutinating antigen. In comparing its haemagglutination properties with the whole cell antigen, the M9 protein showed somewhat less sensitivity starting after the first week post infection with M. gallisepticum strainsand reaching the maximum reactivity at the seventh week post infection, while the whole cell antigen reached its maximum reactivity at the sixth week post infection. Regarding the specificity, both antigens are highly specific and showed no reactions with the M. synoviae antisera. The advantages of M9 protein as haemagglutinating antigen is that it could be used in different areas and is cheaper in preparation

Effect of oxyethylene chain length of the surfactant on the formation of microemulsion

A series of phase diagrams were established to determine the effect of the oxyethylene chain [OE] length of the surfactants on the formation of the microemulsion. Eumulgin B1 [12-OE], Eumulgin B2 [20-OE] and Eumulgin B3 [30-OE] were selected as surfactants in this study. The cloud point of the surfactant increased with the increase of its oxyethylene chain length. Phase studies have indicated that the configuration of the microemulsion depends on the length of OE chain, while the order of addition of the ingredients has only significant effect in case of Eumulgin B3

Optimization of microencapsulation of theophylline with eudragits: part II-effect of additives on microencapsulation of theophylline with eudragits

Aerosil-200 and calcium stearate were tried to enhance microencapsulation of theophylline prepared under optimal conditions. The prepared microcapsules were evaluated by: the physical characteristics, determination of the extent of microencapsulation, theophylline content, flowability of the microcapsules, and in-vitro dissolution of theophylline. The data of dissolution of theophylline from each type of microcapsules were kinetically treated