[Maternal nexposure on collagen type IV pulmonary changes in mouse offspring's]

In this study we evaluated the effect of maternal nicotine administration during pre and postnatal period on collagen IV changes in lung of mouse newborns. Female Balb/C mice were mated and finding vaginal plug was assumed as day zero of pregnancy. Pregnant mice, were divided into 2 experimental and 2 control groups. Experimental group 1, received 3 mg/kg nicotine intrapritoneally from day 5 of gestation to last day of pregnancy. Experimental group 2 received the same amount of nicotine during the same gestational days as well as the first two week after birth [lactation]. The control groups received the same volume of normal saline during the same periods. At the end of exposure time, all of newborns [experimental and control] were anesthetized and their lungs were removed and immunohistochemical study for tracing collagen were carried out. Our finding indicated that collagen reaction in the bronchial basement membrane [BBM] and extra cellular matrix [ECM] of lung parenchyma in experimental increased significantly in comparison to control groups. Cell necrosis definition in lung parenchyma of experimental group 2 were the other finding that our investigation achieved. These data indicate that maternal nicotine exposure may induce a noticeable increasing collagen reasonable in BBM and ECM of respiratory system of next generation. The lungs of these animals which were exposed to nicotine via the placenta and mother's milk, are more susceptible to damages such as abnormal collagen synthesis and cell necrosis

lmmunohistochmistry Study of Collagen IV Changes in Glomerular Basement Memebrane During Fetal and Postnatal Periods of Balb/cMice

In this investigation specific antibody type IV collagen has been used in light microscopy to study development of BMG of the embryonic and postnatal mouse glomerular mesangium. 20 female Balh/C mice were selected randomly and were kept under normal condition, finding vaginal plug was assumed as day zero of pregnancy. 12 pregnant mice were scarified by cervical dislocation in one of gestational days 13-18 and their fetuses were fixed, strially sectioned and iiiimunohistochemistry study for tracing of collagen type IV in BMG was carried out. The same processes were used for kidneys preparation on 5, 10, 15 and 20 postnatal days newborns of 2 mothers for each day. This study indicated that Collagen IV showed weak reaction on day 15 of gestittion. The amount of collagen increased continuously until next days of fetal life and primary of 10 days postnatal in BMG after this Period, collagen IV reaction was not showed significant change in newborns. These data indicate that collagen IV appear just during the glomerular vasculogenesis and because of continuity with vasculature which is required for glomerular endothelial cell differentiation type IV collagen, is the majour structure protein in BMG implicated in these processes

[Study of expression type IV collagen during mouse kidney ubulogenesis in Balb/c mouse]

In this study specific antibody have been used for tracing type IV collagen basement membrane during renal tubules morphogenesis in mouse fetuses. 20 female Balb/C mice were selected randomly and were kept under normal condition, finding vaginal plug was assumed as day zero of pregnancy. 12 pregnant mice were sacrified by cervical dislocation in one of gestational days 13-18 and their fetuses were fixed, serially sectioned and immunohistochemistry study for tracing of collagen type IV in BMG was carried out. The same processes were used for kidneys preparation on 5, 10, 15 and 20 postnatal days newborns of 2 mothers for each day. Based on our data, Collagen IV showed weak reaction on day 14 of gestation in tubular BM. The amount of collagen increased continuously until next days of fetal life and primary of 5 days postnatal in BM. After this period, collagen IV reaction was not showed significant change in newborns. These results indicate that developmental changes in various nephron segments from most immature stages to most differentiated structures are dependent to the type IV collagen expression