Restoration of 2,3 diphosphoglycerate and adenosine trisphosphate concentrations in stored blood by using alanine and/or phosphoenol pyruvate

The effect of incubating dog red cells in citrate-phosphate dextrose adenine [CPDA] containing L-alanine [ALA] and/or phosphoenol pyravate [PEP], at 37°C for one hour was studied. All the additive mixtures increased 2, 3 diphosphoglycerate [2, 3 DPG] level as compared to CPDA control. CPDA-ALA-PED, CPDA-PEP and CPDA-ALA increased 2, 3 DPG level by 60%, 47% and 10% respectively. Also, CPDA-ALA-PEP and CPDA-PEP increased adenosine triphosphate level by 33% and 23% respectively. Storage of blood in the different additive showed that CPDA-ALA-PEP surpassed all other additives in maintaining 2, 3 DPG and ATP concentrations, and decreasing supernatant hemoglobin [Hb] and percent hemolysis, throughout 42 days at 4°C