Heterocycles from 2-Amino-4-[3,4,5-trimethoxy-phenyl]-Naphtho[2,1-b]-4H-pyran-3-carbonitrile

The TITLE compound 1 was prepared and subjected to react with different electrophilic reagents such as phenyl isothiocyanate, benzoyl chloride, 2-cyanocinnamonitrile, ethoxymethylenediethyl- malonate, formamide and carbon disulphide to give the naphtha-pyranopyrimidine derivative 3, naphtha [2,1-b]-4H-pyran derivative 7-9, naphtha [2,1-b]-4H-pyranopyrimidine 10 and naphtha [2,1-b]-4H-pyrano-1,3-thiazine derivative 11, respectively. Treatment of 3 with ethyl bromoacetate and hydrazine hydrate afforded 4 and 5. The hydrazine derivative 5 reacted with benzoylacetone and yielded the pyrazole derivative 6. The IR, [1]H-NMR and Mass spectra of the synthesized compounds were discussed

Detection and partial sequence identification of grapevine leafroll-associated virus-1 in Egypt

Grapevine leafroll-associated virus 1 [GLRaV-1] was detected in the grapevine plants collected from different cultivated areas in Egypt and tested using different serological and molecular tools. Double-antibody sandwich ELISA [DAS-ELISA] was successfully carried out using GLRaV-1 polyclonal antibodies to detect infected plants. PCR with primers designed at the heat shock protein 70 [HSP70] gene region, a fragment of 271 bp of GLRaV-1, was used. Molecular hybridization with non-radioactive probes was used to detect the presence of virus particles. The partial sequence of HSP70 fragment from the Egyptian isolate of GLRaV-1 was performed and showed high identity [95%] with the Australian isolate of GLRaV-1 sequence. The molecular methods used for viral diagnosis showed a higher sensitivity in the detection of GLRaV-1 compared to DAS-ELISA. These procedures may serve as an alternative method for GLRaV-1 detection, due to the weak sensitivity of ELISA test to differentiate between the different isolates

Osteodystrophia fibrosa in native Egyptian goats

Clinical, radiographic, biochemical and pathologic features of osteodystrophia fibrosa were described in 9 native Egyptian goats of different ages [0.5-3 years]. The affected animals were suffering mainly from being off-food together with a visible bilateral, soft, nonpainful swelling of the maxilla and /or m and ible. Radiography of the affected goats revealed generalized loss of bone density involving both cortical and cancellous bone tissue, with thin cortices. Widespread areas of rarefaction particularly in the m and ible were present. Serum biochemical evaluation showed increased alkaline phosphatase, alanine aminotransferase [ALT], aspartate aminotransferase [AST], urea and creatinine with low calcium and phosphorus levels. Osteodystrophia fibrosa was confirmed histologically