Potential role of cytokines as predictors in infection induced premature rupture of membranes; I-colony stimulating factor one [CSF -1] in premature rupture of membranes whether term or preterm

Acute bacterial intraamniotic infections are an important cause of perinatal morbidity and death. Although early diagnosis and aggressive treatment are believed to decrease the ill effects of intraamniotic infection, this goal is frequently difficult to achieve because the infection may not be clinically apparent before delivery in all patients. In this respect, the current study was designed to assess the potential role of CSF-l as a predictor of infection induced rupture membrane whether term or preterm. Collectively 75 amniotic fluid samples were obtained through caesarian section [C.S.] route of delivery. Fifty of them were from women suffering from premature rupture of membrane [PROM] [25 preterm and 25 full term] as well as 25 full term women with intact membranes served as controls. Bacterial cultures, chlamydial LPS antigen, mycoplasma and CSF-l detections revealed a significant association of bacterial infections and CSF-l with PROM deliveries [term and preterm] compared to the controls. A significant increase in CSF-l values were observed among PROM cases compared to the controls. CSF-l values were higher among preterm cases than full term ones and among positive amniotic fluid bacterial culture cases compared to negative culture ones. The highest CSF-l values were with ordinary bacterial infections and the least were with mycoplasmal and ureaplasmal ones

Potential role of cytokines in infection induced premature rupture of membranes; II- interleukin-6 in the amniotic fluid in cases of premature rupture of membranes whether term or preterm

The study aimed at evaluating the role of IL-6 in infection induced premature rupture of membranes [PROM]. 75 pregnant women at labor were the subjects of the study. Group I, included 25 preterm PROM cases, gp II included 25 full term PROM cases and group III included 25 full term with intact membranes [controls]. Amniotic fluid was collected through caesarean section route of delivery. Bacterial culture, chlamydial LPS antigen, mycoplasma detection and IL-6 quantitation, all except mycoplasma revealed, significantly higher values among PROM cases compared to the controls. They revealed to be good diagnostic tools to assess the microbial state of amniotic fluid. IL-6 recorded a significant constant level of 1050 pg/ml among all preterm PROM cases regardless the bacterial results. We conclude that lL-6 determination could be a good predictor of infection induced PROM

Potential role of cytokines in infection induced premature rupture of membrane; III- interleukin one beta in premature rupture of membranes whether term or preterm

The study was conducted upon 75 pregnant women delivering by caesarean section [CS] route. Group I [n=25] included preterm premature rupture of membranes [PROM] cases [20-37 wks of gestation], Group II [n=25] included full term PROM cases [38-42 wks], Group III [n=25] included the controls who were full term with intact membranes submitted to elective CS. Routine bacteriologic culture, chlamydial LPS and mycoplasmal detection and IL-1beta estimation of amniotic fluid samples revealed a significantly elevated IL-1beta values among preterm PROM cases with positive bacterial culture compared to negative culture cases. Also full term PROM cases revealed significantly elevated IL-1beta values among positive chlamydial amniotic fluid samples. To conclude IL-1beta could be a potential marker in infection induced PROM cases. Further studies arc warranted to clarify the role of IL-1beta receptor antagonist as a causal therapy in PROM cases