Mutagenicity of the mycotoxin diacetoxyscirpenol on somatic and germ cells of mice

The study was conducted on both somatic and germ cells in addition to the sperm morphology analysis. Diacetoxyscirpenol [DAS] treatment resulted in a highly significant reduction [P <0.01] in mitotic activity at all the doses tested, confirming that DAS is a potent protein and DNA synthesis inhibitor. In the somatic cells [bone marrow], the observed chromosome abnormalities were chromatic gaps, breaks, centromeric attenuation and endomitosis. At single dose experiment, significant abnormalities were observed only after high dose treatment, they mainly consisted of chromatoid gaps, centromeric attenuation and endomitosis [4.2 + 1.3, 4.2 +/- 2.4, respectively] and aneuploids were also observed [5.8 +/- 1.6]. In contrast, at the repeated dose experiment similar abnormalities were also observed with some significance, but no systematic relation could be settled. The present results confirmed a positive correlation between cytogenetic damage and sperm abnormality. The results also proved that DAS is a very toxic mycotoxin. The most important severity of it, in addition to including chromosomal abnormalities, is its inhibition of DNA synthesis and subsequently affecting the cell cycle and division

Radiotherapy, methotrexate, and topical keratolytic in treatment of psoriasis in a comparative study

In this study eighteen psoriatic patients were treated by various lines of therapy [local keratolytic, systemic methotrexate and radiotherapy] in order to evaluate the pathological changes in psoriasis under different therapies by cell counting study pre - and post-treatment. It was found that there were insignificant statistical difference in the different type of cells before and after using local therapy, while, there were statistical significant reduction in the percentage of mitotic cells after methotrexate. Also, there were very highly significant and highly significant reduction in the percentage of mitotic and resting cells respectively after radiotherapy. While, there was very highly significant statistical increase in the percentage of degenerating cells after radiotherapy