immediate enzymatic cocktail effect on the absorptive function of neobladder made by ileocystoplasty

The effectiveness of an enzymatic cocktail, collagenase and trypsin, on the absorptive function of ileal segment was studied to evaluate the changes within groups after performing ileocystoplasty in an animal model. Twenty-one female mixed-bred Persian dogs were randomized into 7 groups. The groups included: 1] negative control group [NCG] in which no ileocystoplasty was performed [n = 3]; 2] positive control group [PCG] in which routine ileocystoplasty was performed [n = 3] and groups 3 to 7] those underwent ileocystoplasty with the ileal segment being treated with collagenase and trypsin for 5, 10, 15, 20 or 25 min [groups, 5 minutes enzymatic treatment group [5METG], 10METG, 15METG, 20METG and 25METG; each containing three dogs]. To perform the glucose absorption test, 50% dextrose was instilled into the bladder five weeks after surgery. The blood glucose level [BGL] was measured in each group before the study [T1], two weeks after the surgery in PCG and other treatment groups [T2], before anaesthesia [T3], after anaesthesia at 5-minute intervals up to 25 min [T4 to T9, respectively]. There was no significant difference in BGL in T1 to T3 and T4 to T9 in 25METG and NCG, however, BGL was found significantly higher in T1 to T3 and T4 to T9 in PCG, 5METG, 10METG, 15METG and 20METG. It can be concluded that collagenase and trypsin can affect absorptive function of the neobladder and are able to reduce the absorptive function, particularly in early days after the surgery. Moreover, these results also confirm that 50% dextrose instilled into the neobladder and native urinary bladder will not increase BGL in 25METG and NCG even with increasing the time of exposure of neo- or native urinary bladder to enzymatic cocktail till 25 min

[Experimental vesicouereteral reflux induction in dogs]

Objective: To induce experimental vesicoureteral reflux in dogs

Design: Experimental study

Animals: A total of 16 mixed- breed Persian dogs with diffrent sex

Procedure: All sixteen dogs evaluated for absence of congenital or acquired vesicoureteral reflux before study by cystography. Vesicoureteral reflux induction was attempted in healthy animals by ureteral unroofing [3-5 mm] without suture, unroofing [3-5 mm] with catheter as a cutting guide and suturing by silk, unroofing [3-5 mm] with catheter and suturing by nylon and unroofing [5-7 mm] with catheter and suturing by nylon. After ten days the reflux assessment was confirmed by cystography

Statistical analysis: Descriptive study

Results: Vesico ureteral induced refluxes in all four groups were variable [incision length, catheter as guide, Number and kind of suture material]but refluxes induced with 5-7 mm incision length and nylon suturing was excellent as compared with other three groups

Clinical implications: Cutting method with catheter and nylon suture material used for vesicoureteral reflux induction in dogs, were preferred with special attention to be made to the length of cutting and the number of suture needed