Effect of different magnetic poles on hemoglobin-oxygen affinity in hemodialyzed patients

In this study the influence of static magnetic fields, from north, south and both north and south poles, on the hemoglobin affinity for oxygen in chronic hemodialyzed patients was investigated as a trial to overcome dialysis induced hypoxemia and dialysis associated with a rise in hemoglobin affinity for oxygen as a result of alkalizating agents. The results were given

affinity of hemoglobin and its components to lead, in occupationally exposed workers

The present study was carried out on 225 workers occupationally exposed to lead in the printing industry. Blood-lead concentration was measured by atomic absorption spectroscopy. The level increased with the severity of the toxicity. Chromatographic separation followed by determination of lead concentration, showed that fetal hemoglobin [HbF] possesses a higher affinity to lead than HbA and HbA2. B-chain represented the main lead receptor of adult hemoglobin [HbA]. While, alpha-globin of fetal origin had a higher affinity for lead. Viscosity and conductivity of Hb were increased with blood-lead elevation

pH-dependent structural change of cytochrome-C

Effect of pH variation on the dynamic motion of cytochrome-C through conductivity, electronic absorption spectra and magnetic susceptibility coupled with molecular weight change were investigated. The very low pH region increases the probability of conversion native cytochrome-C to random coil. Neutralization leads to fold protein after being unfolded. Low spin state, mixture of low spin and high spin and high spin state of iron heme of cytochrome-C were demonstrated at acidic, neutral and alkali solution, respectively

Vitamin D metabolism among Egyptian infants and children affected with beta thalassemia

Sixty-five patients suffering from beta-thalassemia major were studied [46 males and 19 females]. Their ages ranged between 6/12-12 years [mean 5.8 +/- 2.2 years]. Twenty-five children of comparable age, sex, and socioeconomic status were studied as controls. Patients were classified into newly diagnosed and long-standing, who were furtherly classified into cases without hyperspienism, cases with hypersplenism and splenectonized cases. Each patient was subjected to full history taking thorough clinical examination and determination of serum calcium, phosphorus, alkaline phosphatase both total and fractional isoenzymes [liver and bone], 25 [OH] vitamin D3 1, 25 [OH]2 vitamin D3 parathyroid hormone and urinary calcium excretion. Serum ca1cium was significantly decreased after splencctomy. Serum phosphorus was significantly decreased except in patients with hypersplenism who showed normal values. Parathyroid hormone was decreased after splenectomy. 25 [OH]2 VD3 showed high significant decrease except in patients with hypersplenism. 1, 25 [OH]2 VD, showed very high significant decrease in all patients. Calcium/creatinine ratio in urine showed a very high significant increase in all cases. It might be con eluded that follow up of the levels of serum Ca, P, 25 [OH] VD3 1, 25 [OH]2 VD3, and alkaline phosphatase should be carried out in all thalassemic patients for early supplementation with vitamin D to avoid any bone mineralization defect together with the regular follow up of parathyroid hormone level especially after splenectomy