Comparative serodiagnostic tests for toxoplasmosis among sheep and goats in Giza governorate, Egypt

Toxoplasma gondii, is a ubiquitous apicomplexan parasite of warm-blooded animals. intracellular protozoan and is one of the most common parasitic infections of human, hi the present study diagnosis of Toxoplasma gondii was directed towards the serological methods including enzyme-linked immunosorbent assay [ELISA], and indirect hemagglutination assay [IHat]. The comparative serological examination of 320 sheep and 306 goats blood serum antibody body titres against Toxoplasma gondii were analyzed at 10 settlements in the Giza region, Egypt. The IHat showed the higher prevalence of toxoplasmosis 47.5% in sheep and 59.5%in goats respectively with antibody titres ranging front 1:20 and 1:640'. Also ELISA seropositive samples 44% in sheep and 55.5% in goat respectively. It could be concluded that the IHAT and ELISA tests are an efficient diagnostic tools for detection and selective diagnosis of Toxoplasmosis in sheep and goats using local strain isolate in Egypt

Molecular identity of the electropharetic pattern and the major cross-reacting immunogens of toxoplasma gondii [RH strain] and neospora caninum

The complex electrophoretic profile of the antigenic components of Toxoplasma gondii and Neospora caninum was studied. T. gondii [RH strain] trophozoite's antigens were resolved by gel electrophoresis under denaturating condition. Resolved polypeptides were tested by immunoblotting against hyper-immune serum prepared in Boscat rabbits. Immunoblot analysis revealed 6 reactive bands, which are 158, 111, 102, 86, 55 and 33 kDa, out of 10 antigenic bands of the parasite. Concerning N. caninum trophozoite's antigens, immunoblot analysis revealed 5 reactive bands, which are 159, 119, 106, 97 and 39 kDa, out of 8 antigenic bands of the parasite. Cross reactivity trials between the 2 parasites explore 3 cross-reactive bands, indicating high antigenic similarity in between