ABSTRACT
To compare the skin responses of infected andnon-infected sheep with Linguatula serrata nymph.Experimental study.Thirty native sheep.The nymphs of Linguatula were collectedfrom mesenteric lymph nodes of slaughtered animals andthe protein of nymphs was extracted. The extracted proteinwas injected at the dose of 0.2 ml intra-dermopalpebrallyinto the skin of lower eyelid of sheep. A placebo wasinjected into the skin of contralateral eyelid. The skinreaction was evaluated 1 hour after injection. Infectionstatus of injected sheep was determined by demonstrationof Linguatula serrata nymphs in the mesenteric lymphnodes after slaughter as gold standard.Compute sensitivity, specificity, accuracy, positiveandnegativepredictivevalues, likelihood ratio and kappa statistic of the intradermal skintest at a different cut off values.Ourcalculatedmeasuresofdiagnosticperformance of intradermal skin test of Linguatula serrate were lower than anticipated and those previously reported.Intradermal skin test can be used for detectionof non-infected sheep