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Journal of the Egyptian Society of Parasitology. 2006; 36 (3): 795-807
in English | IMEMR | ID: emr-78333

ABSTRACT

Electrophoresis of plasma protein of B. alexandrina [uninfected and infected with S. mansoni] showed that the major dominant bands had molecular weights of 20,44,96,139 and 205KD in both types of snails. The 1day and 1 week post miracidia1 exposure [PME] groups were characterized by band 54 KD. All groups except a day PMA were characterized by a common band of MW 65 KD. Three days PME group had three bands of 123 KD, 150 and 177 KD, not found in other groups. The highest similarity index in 2 weeks PME and 5 weeks PME groups [during cercaria1 shedding] was 0.667 the lowest one was in 3-dys PME [0.5]. the 3-days PME had a unique band of MW 177.04 KD, not found in other groups. Similar electrophoretic pattern of B. alexandrina protein was seen. The major dominant bands had molecular weights of 14, 21, 80 and 140KD in both non-infected and infected snails. The 1day PME had a band of 48.483 KD, 3days PME had band of 87.985 KD, one-week PME group characterized by two band 61.761 KD and 70.33 KD. The two-weeks PME had a band 91.111 KD. While, the 5week PME [during cercaria1 production] was the only group that shared the common band of MW 115 KD with controls. The highest similarity index in 5 weeks PME [during cercaria1 shedding] group was 0.545 and the lowest one was in 1 week and 2 weeks PME [0.43]. The electrophoresis of plasma protein of B. truncates [uninfected and infected with S. haematobium] showed that the major dominant bands had molecular weights of 20, 30, 65, 80, 106, 117 and 170 KD in both type of snails. The 1 day PME group was characterized by three band of MWs 26.539, 51.891 and 91.509 KD. All experimental groups, except 5 weeks PME [during cercaria1 shedding] and control, had a common band of MW 45KD. Three days PME group had a characteristic band of 113.72 KD which was not found in any other group. The highest similarity index was in one week PME group was 0.857 and the lowest one in 1 days PME [0.5]. In B. truncatus tissue protein, the major dominant band by electro- phoretic pattern had molecular weights of 20, 45, 54, 80, 97, and 171KD in both type of snails. A day PME had of 73.544KD and a week PME had a band of MW 60.813 KD. Two and 5weeks PME groups had 2 bands of MWs 27 and 62 KD .All experimental groups had a characteristic band not found in control of MW 141 KD. The highest similarity index in 3-days PME was 0.8 and the lowest one was in 5 weeks PME during cercaria1 shedding [0.545].


Subject(s)
Hemolymph , Bulinus , Schistosomiasis mansoni , Snails
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