ABSTRACT
Introdução: Pinos intrarradiculares são recomendados para melhorar a retenção de coroas artificiais e distribuir forças intrabucais ao longo da raiz. Se o espaço criado pela remoção parcial da obturação não for preenchido adequadamente, pode ocorrer uma infiltração maciça de microrganismos da cavidade bucal. Objetivo: O objetivo do presente estudo foi avaliar a eficácia contra a infiltração bacteriana de uma barreira intracanal colocada diretamente sobre o remanescente da obturação do canal radicular, após o preparo de espaço para o pino. Métodos: Setenta e dois dentes humanos unirradiculares foram instrumentados, obturados e divididos aleatoriamente em três grupos experimentais e dois grupos controle. O grupo 1 não recebeu tratamento adicional após a obturação e o preparo do espaço, enquanto os Grupos 2 e 3 receberam uma barreira composta por material de selamento temporário com 1,0 e 2,0 mm de espessura, respectiva- mente. Uma cultura de Enterococcus faecalis foi inoculada nos espaços preparados para receber o pino intrarradicular, a cada três dias, por um período de 60 dias. A infiltração foi avaliada diariamente pela turbidade do meio de cultura. Resultados e Conclusão: Houve infiltração bacteriana nos três grupos experimentais, sendo significativamente maior e ocorrendo mais rapidamente no G1 (p<0,05), em comparação aos G2 e G3. Houve infiltração em todos os grupos controles positivos; porém, nenhuma infiltração foi observada nos grupos controles negativos. Não foi encontrada diferença significativa (p>0,05) entre o G2 e o G3, em relação à taxa e ao período de infiltração. Pode-se concluir que a barreira reduziu a incidência de infiltração e atrasou o tempo de ocorrência (AU).
Introduction: Intraradicular posts are recommended to improve the retention of artificial crowns and to distribute intraoral forces along the root. If the space created by partially removing the obturation is not adequately filled, it can lead to a massive infiltration of microorganisms from the oral cavity. Aim: The aim of this study was to assess the influence on bacterial infiltration of an intracanal barrier placed directly over the remaining root canal filling following post space preparation. Material and Methods: Seventy-two human single-rooted teeth were instrumented, filled, and then randomly divided into three experimental groups and two control groups. Group 1 received no additional treatment after filling and post space preparation, whereas Groups 2 and 3 received a barrier composed of temporary sealing material 1.0- and 2.0-mm thick, respectively. A culture of Enterococcus faecalis was inoculated into the spaces prepared to receive the intraradicular post, every three days over a 60-day period. Infiltration was evaluated daily by observing the turbidity of the culture medium. Results and Conclusion: Bacterial infiltration was found in all three experimental groups, but was significantly greater and occurred more quickly in G1 (p < 0.05), compared with G2 and G3. Infiltration was observed in all positive controls, whereas no infiltration was found in the negative controls. No significant difference (p > 0.05) was found between G2 and G3, regarding the rate and time frame of infiltration. It can be concluded that the barrier reduced the incidence of infiltration, and delayed the time of occurrence (AU).
Subject(s)
Humans , Root Canal Obturation , Enterococcus faecalis , Dental Pins , Efficacy , CrownsABSTRACT
ABSTRACT Objective: evaluate the sterilization of human teeth irradiated by microwaves. Methods: Sixty human third molars are divided into three groups (n = 20): G1 without sterilization (negative control); G2 - autoclaving for 20 minutes 1Kgf/cm2 at 120 ° C (positive control); G3 - sterilization in a microwave vessel containing 200ml of distilled water in a microwave irradiated at 650W for 3 minutes. Results: No culture media of G2 and G3 presented contamination after autoclaving and microwave sterilization. Conclusion: Autoclave sterilization and microwave sterilization were effective decontamination methods under the experimental conditions tested.
RESUMO Objetivos: avaliar a esterilização de dentes humanos através do uso da autoclave e da irradiação por micro-ondas. Métodos: Quarenta incisivos centrais superiores humanos foram distribuídos em três grupos: G1- sem esterilização (controle negativo); G2 - esterilização em autoclave, durante 20 minutos, 1Kgf/cm2, a 120ºC (controle positivo); G3 - esterilização com micro--ondas em recipiente contendo 100ml de água destilada, irradiado a 900W em microondas durante 3 minutos. Resultados: Nenhum meio de cultura do G2 e G3 apresentou contaminação após esterilização em autoclave e microondas respectivamente. Conclusão: A esterilização em autoclave e a esterilização em micro-ondas foram métodos eficazes de descontaminação nas condições experimentais testadas.
ABSTRACT
To assess the sealing capacity of different dental cements regarding apical microleakage on teeth with prefabricated posts. Material and Methods:Forty-eight single-rooted teeth were selectedand, after having their coronal part removed at 10mm standard, underwent endodontic cleaning and preparation. The space for the post was prepared with # 3 Largo bur at 7 mm standard value. The study included the following groups: Group I, post prefabricated with # 1 intracanal glass fiber cemented with zinc phosphate cement (n = 12); Group II, post prefabricated with # 1 intracanal glass fiber cemented with conventional glass ionomer cement, Vidrion C (n = 12); Group III, post prefabricated with # 1 intracanal glass fiber cemented with chemical action resin cement, Multilink (n = 12). Two groups of six teeth each were also included, one as negative control group and the other as positive control. After cementing, teeth were tested through a dual-chamber experimental model, using Enterococcus Faecalis for apical contamination. Results:The control groups behaved as expected: positive control group with total contamination in 11 days and negative control group without any contaminated specimen within observation time of 60 days. Group I showed microleakage in all specimens for up to 29 days (100%)...
Group II and Group III showed microleakage in 50 and 49 specimens (83.33% and 75%) respectively along the research time of 60 days. Group 1 showed microleakage in all specimens for up to 29 days (100%). Group 2 and Group 3 showed microleakage in 50 and 49 specimens (83.33% and 75%) respectively along the research time of 60 days. The Log-Rank test showed differences between Groups 1 and 2 (p<0.05) and between Groups 1and 3 (p<0.05). However, there was no difference between Groups 2 and 3 (p> 0.05). The results showed higher delay in bacterial infiltration in Groups 2 and 3 when compared to Group 1.Conclusion:No cement completely prevented infiltration during the experimental period. Zinc phosphate cement had the lowest apical sealing capacity while glass ionomer cement and resin cement showed similar results...
Subject(s)
Humans , Dental Cements/chemistry , Glass Ionomer Cements , Dental Leakage , Tooth Apex/microbiology , Brazil , Endodontics/methodsABSTRACT
Objective: to evaluate the ability of different MTA thicknesses (3 and 5 mm), with or without conventional fillings, in preventing bacterial infiltration of Enterococcus faecalis in teeth with incompletely formed apices. Material and Methods: apical barriers were created which 3- and 5-mm-thick of MTA with and without conventional filling of the remaining root. 48 single-rooted human teeth were divided into four experimental groups (n=10) and two controls groups (n=8). After being mounted on a testing apparatus, Enterococcus faecalis was used as an indicator of bacterial infiltration. Inoculations were renewed every 3 days during a period of 60 days, and infiltration was assessed daily by checking the culture medium for turbidity. The results were analyzed using descriptive and analytical statistics (survival analysis). The level of significance was set at 5%. The tests were performed using the software GraphPad Prism 5.00. Results: the positive control group infiltrated within 24 hours, while the negative control group, no infiltrated. The Long-rank test did not demonstrate the existence of differences between groups. However, the Long-rank test for tendencies showed of a tendency to retard the infiltration. Conclusion: none of the MTA barriers were effective in preventing bacterial infiltration. The gutta-percha filling of the root segment in association with the 5-mm apical barrier of MTA showed a tendency to delay bacterial infiltration.
Subject(s)
Humans , Dentition, Permanent , Enterococcus faecium/immunology , Dental Leakage/diagnosis , Dental Leakage/etiology , Brazil , Radiography, Dental/instrumentation , Survival AnalysisABSTRACT
A literatura é unânime em afirmar que o uso de aparelhos fixos ortodônticos para a correção da má oclusão causa uma mudança da microbiota bucal. Há um aumento do acúmulo de placa e de Streptococcus mutans, microrganismo associado à desmineralização do esmalte dentário e desenvolvimento da lesão de cárie. Dependendo do tipo de material dos acessórios ortodônticos, sugere-se uma adesão e colonização bacteriana diferenciada. Diante das divergências encontradas na literatura, este artigo tem como objetivo realizar uma revisão crítica sobre a adesão de microrganismos em aparelhos ortodônticos fixos e sua consequência na cavidade bucal.
It is a common sense in the literature that the use of orthodontic appliances for malocclusion correction causes a change in the oral microflora. There is an increase in plaque accumulation and an enhancement of Streptococcus mutans colonization, which is related to enamel demineralization and caries progression. For each type of material used in the appliances, there will be a specific bacterial colonization. In order to clarify all the divergences found in the literature when colonization is studied, the aim of this paper is to make a critical review of the literature concerning microorganism adhesion in fixed orthodontic appliances and the consequences in the oral cavity.