ABSTRACT
We investigate the effects of training and detraining on the satellite cell activation in thoroughbred horse muscles after an exhaustive exercise. Six horses were subjected to conventional training for 18 weeks and detraining for 6 weeks. Before training (Pre), after 10-week and 18-week training (10Tr and 18Tr), and after 6-week detraining (DTr), an incremental exercise test (IET) was performed on inclined treadmill to measure VO<sub>2</sub>max and the velocity at a plasma lactate of 4 mmol/l (VLA4). Biopsy samples from gluteus medius muscle was obtained before and at 1 minute (1min), 3 hour (3hr), 6 hour (6hr) and 1 day after each IET. Number of muscle satellite cell were counted in type identified muscle fibers by immuno-histochemical stain images. The levels of mRNA expressions were determined using real time RT-PCR system. The number of satellite cells in 10Tr was significantly higher in type IIa fibers (0.31±0.10) than Pre (0.15±0.06). As compared to each value before IET, IL-6 mRNA expression (fold change) increased remarkably at 6hr after IET in 10Tr (x 2290.2) and 18Tr (x 2304.2), but not in both Pre (x 260.0) and DTr (x 853.3). IGF-I and Myogenin mRNA expressions were significantly increased at 1 day after IET in 18Tr (x 6.6 and x 3.3), but not in both Pre and DTr. These results suggested that the increased reactivity of satellite cells by training for 18 weeks is almost disappeared after detraining for 6 week, as well as VO<sub>2</sub>max and VLA4.
ABSTRACT
The thoroughbred horse is a remarkable animal, with both speed and endurance abilities during running. Muscle adaptation associated with training and growth has been examined using various physiological, histochemical and biochemical measurements. We have emphasized the importance of type IIX fiber recruitment in training program for thoroughbred. Because type IIX fiber provides the greatest contribution to total muscle volume in the gluteus medius muscle and increases in oxidative enzymes of the fibers would markedly improve the whole-muscle oxidative capacity. Based on these concepts, we examined the adaptations of type IIX fiber including hybrid fiber to various training programs.It is considered that scientific information about optimal training of these excellent abilities can provide significant insights into exercise physiology and health science not only for thoroughbred horses, but also for human beings.