ABSTRACT
Objective: To assess the effects of anastrozole use as hormonal treatment of breast cancer on the serum estrogen level in an animal model
Study Design: Laboratory based randomized controlled trial
Place and Duration of Study: Department of Anatomy, Army Medical College, Rawalpindi in collaboration with National Institute of Health, Islamabad, 7 months from Jun 2012 to Nov 2012
Material and Methods: Thirty adult female rabbits [New Zealand] average weight 1.2 to 2 kg and age between 6 months to 2 years were selected. Ten were kept in control group A and 10 were kept in experimental groups B and C each. Group B was given anastrozole in the dose parallel to normal human dose and group C was given a dose 10 times higher than the normal dose for six months. After the completion of the study blood estrogen levels were taken to evaluate serum estrogen level of the groups. The results were compared among the groups for statistical significance by using SPSS version 21
Result: After maintaining a steady dose of anastrozole in both experimental groups for six months, blood samples were taken to calculate the mean serum level of estrogen in each group the normal level was 186.1 +/- 63.59 pmol/L in group A and 116.4 +/- 48.58 pmol/L and 108.2 +/- 20.40 pmol/L in experimental groups B and C respectively. The p-value was 0.008 which was statistically significant. The p-value between the control and experimental group C was 0.003 which is also highly significant. The comparison of the serum estrogen levels of both the experimental groups B and C was found to be statistically insignificant
Conclusion: Adminstration of normal dose of anastrazoledecreases serum estrogen levels. This decrease is significant statistically. Increasing the dose of anastrozole decreases the serum estrogen levels but this decrease is not significant statistically
ABSTRACT
Background: Athletes use anabolic androgenic steroids in excess of normal therapeutic levels to improve their stamina and performance, and this apparently describes the increase in their toxic side effects
Objective: To determine the ameliorative effects of Pomegranate Juice and Pomegranate Peel Extract on steroid induced inflammation in mice kidneys
Methodology: Study Design: Laboratory based Randomized control study
Duration and Place of Study: Department of Anatomy, Army Medical College, Rawalpindi, in cooperation with National Institute of Health [NIH], Islamabad, from 1[st] May to 30[th] June 2015. Forty healthy male and female BALB/c mice with average weight of 25-30 gms were randomly distributed into four groups, each group with five male and five female mice. Group A worked as a control group and groups B, C and D worked as experimental groups. Mice in experimental groups B,C and D were given a single injection of [Nandrolone decanoate] ND intramuscularly, at the dose of 1 mg/100 gm body weight, in the hind limb once weekly for 8 weeks. Mice in experimental group C was also given pomegranate juice [PJ] at the dosage of 3ml/kg body weight via oral gavage tube daily for 8 weeks and mice in experimental group D was given pomegranate peel extract [PPE] at the dosage of 200mg/kg body weight via oral gavage tube daily for 8 weeks. The animals were sacrificed after the experimental period was over and kidney tissues were processed for sectioning and staining. The results of all the groups were compiled and compared by using SPSS 21. Comparison of qualitative variables between the groups was done by applying Chi square test
Results: Inflammatory infiltrate were significantly higher in steroid administered experimental group B and statistical improvement was seen when comparison of experimental group B was done with PJ and PPE administered experimental groups C and D, respectively [p=0.001and 0.000]. Comparison of experimental groups C and D showed no statistical significance [p=1.0]
Conclusion: It was concluded that both forms of Pomegranate has ameliorative effects on steroid induced inflammatory changes in mice kidneys