ABSTRACT
The production of murine monoclonal antibody [MAb] has not yet been reported in Iran. The present work describes for the first time the generation of several murine hybridoma clones secreting MAbs directed against human leukocyte surface antigens. The secreted antibodies by hybridoma clones have been screened on different lymphoid and non-lymphoid tissues. Results indicated that of seven hybridomas, clones 3F11, 3C3 and 1F2 showed a strong reactivity for T-cells purified from thymus and tonsil tissues. Moreover, clones 1 D4 and 6G5 which partially stained thymus tissues were found to be negative on purified B-cells and monocytic cell line U937. The third group, 4E5 and 4F4 hybridoma clones was expressed weakly on purified T, B-cells and U937 cell line. Further work is needed to determine the epitopes recognized by these MAbs and a comparison of the data with those presented at the previous leukocyte antigens workshop